胃癌_____]_胃肿瘤；_ras基因；聚合酶链反应

胃癌组织中Hras及Kras基因第12密码子点突变的检测及意义

http://www.100md.com 《青岛大学医学院院报》 2006年第1期

胃癌,,],胃肿瘤；,ras基因；聚合酶链反应,1材料和方法,2结果,3讨论,[参考文献]

     [摘要] 目的探讨人胃癌组织中Hras、Kras基因第12密码子点突变情况及意义。方法收集43例胃癌及相应癌旁组织标本。应用聚合酶链反应限制性片段长度多态性(PCRRFLP)分析法检测Hras、Kras基因第12密码子点突变情况。结果 43例胃癌组织中有1例发生Hras突变(1/43，2.3%)，癌旁组织中未发生突变。Kras在胃癌及癌旁组织中均未发生突变。结论胃癌组织中Hras、Kras基因第12密码子点突变率低，在胃癌的发生中不起主要作用。

    [关键词] 胃肿瘤； ras基因；聚合酶链反应

    DETECTION OF POINT MUTATION OF CODON 12 OF Hras AND Kras IN GASTRIC CANCER TISSUESGU HUALI, DONG JING (Department of Emergency, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China)

    [ABSTRACT]ObjectiveTo investigate the point mutation of codon 12 of Hras and Kras in tissues of gastric carcinoma. MethodsFortythree gastric cancer specimens and paraneoplastic tissues were obtained. Point mutation in codon 12 of Hras and Kras were detected by polymerase chain reactionrestriction fragment length polymorphism (PCRRFLP). ResultsOne case (1/43, 2.3%) of mutation of Hras 12 codon was detected, no mutation was found in paraneoplastic. No mutation of Kras was detected in both cancer and paraneoplastic tissues. ConclusionThe point mutation rate of codon 12 of Hras and Kras is low in gastric cancer, which suggestive of little role in the genesis of the cancer. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/12/96/33/53.htm

您现在查看是摘要页，全文长 7507 字符。