Study of protective effect of interleukinª²10 on ischemia/reperfusionª²induced lung injury following lung transplantation

    ZHOU Yongª²An, LIU Kun, GU Zhongª²Ping, ZHANG Tao

    Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To investigate the protective effects of Interleukinª²10 (ILª²10) on ischemia/reperfusion (I/R)ª²induced lung injury following rat lung transplantation and to explore its mechanisms. METHODS: Thirtyª²six SD rats were randomly divided into 3 groups with 12 rats in each group: sham operation group (control), lung transplantation group and ILª²10 treatment group. ILª²10 was administered to the recipient rats through intraª²trachea way. The wet/dry ratio of lung, lung permeability index and neutrophil percentage in bronchoalveolar lavage fluid (BALF) were detected. The serum level of tumor necrosis factor ¦Á(TNF¦Á)was detected by enzymeª²linked immunosorbent assay (ELISA). RESULTS: The wet/dry ratio of lung, lung permeability index and neutrophils percentage in BALF of I/R group were (6ª±57¡À0ª±89),(23ª±49¡À5ª±13)¡Á10-4 and (49ª±84¡À6ª±53)%, significantly higher than those of the control group(P©‚0ª±01). The administration of ILª²10 to recipient rats before the operation markedly reduced the indexes above to (4ª±23¡À0ª±58), (15ª±96¡À3ª±04)¡Á10-4 and (20ª±33¡À4ª±26)%, respectively(P©‚0ª±01). The serum level of TNF¦Á(¦Ìg/L)increased to (0ª±86¡À0ª±11) after lung transplantation, markedly higher than that of control group, however, the ILª²10 treatment could reduce its level significantly to [(0ª±39¡À0ª±05),P©‚0ª±01]. CONCLUSION: ILª²10 protects the lung function against I/R injury after transplantation, which is related to its inhibition of TNF¦Á release and activation of neutrophils. ......