干扰素Ⅱ型____

BCG对Th细胞免疫调节作用的研究

http://www.100md.com 《齐鲁医学杂志》 2006年第2期

干扰素Ⅱ型,,],BCG；白细胞介素4；白细胞介素5；干扰素Ⅱ型；小鼠，近交BALB,C,1材料与方法,2结果,3讨论,[参考文献]

     [摘要] 目的探讨BCG对小鼠Th1/Th2细胞免疫反应的调节作用，研究BCG是否增强小鼠Th1型免疫反应。方法将实验动物分为对照Ⅰ组、BCG14组、对照Ⅱ组、BCG42组。BCG14组、BCG42组给予BCG每次0.03 mL, 隔日1次腹部皮下注射，对照组注射相同剂量的生理盐水。第14天，将对照Ⅰ组、BCG14组分别处死取脾脏，BCG42组则继续行BCG免疫，至第42天对照Ⅱ组及BCG42组处死取脾脏。用ELISA法测定脾脏单个核细胞(MNCs)培养上清液中IL4、IL5、IFNγ含量。结果 BCG14组、BCG42组IFNγ水平较对照组明显增加(t=3.73、3.86,P<0.01)。BCG14组IFNγ水平与BCG42组比较差异无显著性意义(t=1.68, P>0.05)。其他两项指标与对照组比较差异无显著性(t=1.05～1.17, P>0.05)。结论 BCG能上调小鼠Th1型免疫反应。

    [关键词] BCG；白细胞介素4；白细胞介素5；干扰素Ⅱ型；小鼠，近交BALB C

    EFFECTS OF BCG ON THE REGULATION OF Th CELL IMMUNITY

    LIU WEI, GUO LING, MIAO WENYU, et al

    (Department of Respiratory Diseases, Rizhao Peoples Hospital, Rizhao 276826, China)

    [ABSTRACT]ObjectiveTo investigate the effect of BCG on the regulation of Th1/Th2 balance in mice and explore whether BCG increased type Th1 immune response. MethodsThe experimental animals were divided into Control Group Ⅰ and BCG14 Group; Control Group Ⅱ and BCG42 Group. For BCG14 Group and BCG42 Group, a dose of BCG 0.03 mL was injected subcutaneously every other day and normal saline, same dose, for control groups. On day 14, those in Control Group Ⅰ and BCG14 Group were killed and spleens removed; those in BCG42 Group continued BCG immunity and killed on day 42, and the spleens removed. The levels of interleukin4, interleukin5 and interferonγ of mononuclear cells (MNCs) supernatant were measured by ELISA. Results IFNγ levels in BCG14 and BCG42 groups were significantly higher than those in the control group (t=3.73, 3.86;P<0.01). The difference of IFNγ levels of MNCs supernatant between BCG14 and BCG42 was not significant (t=1.68, P>0.05). As compared with control groups, the difference of the other two items showed no significance (t=1.05-1.17, P>0.05).ConclusionBCG immunization could upregulate Th1type immune response. ......

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