摘 要：目的：观察血液储存时间、温度对FⅧ：C活性的影响，为制备FⅧ：C活性符合质量标准的新鲜冰冻血浆及冷沉淀提供参考依据。方法：采用生物梅里埃公司生产的COAG-A-MATE MTX全自动血凝仪测定30份血液分别于不同温度，不同时间制备的FFP的FⅧ：C活性水平。结果：血液采集后不同储存温度，不同储存时间所制备的FFP中FⅧ：C活性水平有显著性差异(P<0.05)。结论：FFP中FⅧ：C活性水平与储存温度、时间相关，全血采集后4℃冰箱保存，于6h内分离、-50℃速冻而成的FFP其FⅧ：C活性水平符合新鲜冰冻血浆质量要求。

    关键词： 温度； 时间； FFP； FⅧ：C活性

    Effects of Time and Temperature of Blood Storage on Activity of the FⅧ-C

    LIU Ren-qiang, QIN Yan-lan

    (Dongguan Blood Center, Guangdong Dongguan 523930, China)

    Abstract： Objective： To explore the effects of storage time and temperature of blood on function of the FⅧ-C, to offer references on making fresh frozen plasma. Method： Detected 30 fresh frozen samples in different temperature and different time to observe the activity of the FⅧ-C by automatic COAG-A-MATE MTX. Result：The activity of the FⅧ-C in fresh frozen plasma was different in different time and different temperature after blood collecting (P<0.05) . Conclusion： The activity of the FⅧ-C is relative to the storage time and temperature of blood. It is normal if plasma was fast frozen in -50℃ in 6h after blood collection which was stored in 4℃. ......