Vitamin D3对裸鼠乳腺癌生长的影响
胆骨化醇,,胆骨化醇;乳腺肿瘤;细胞凋亡;细胞周期,VitaminD3对裸鼠乳腺癌生长的影响,0引言,1材料和方法,2结果,3讨论,【参考文献】
Effect of vitamin D3 on growth of breast cancer in nude mouse xenograft modelsZHOU Wei, WANG JiaLin, ZHAO YaPing, WANG Yu, ZHANG Yong
Department of Endocrinology, PLA 82nd Hospital, Huaian 223001, China
【Abstract】 AIM: To study the effect of Vit D3 on breast cancer in nude mouse xenograft models. METHODS: Each Balb/c nude mouse was injected subcutaneously with human breast cancer cell line MCF7. Two thousand units of Vit D3 was injected in mice thigh muscle every other day and mice were fed with tamoxifen (5 mg/kg) every day. Forty experimental mice were randomly divided into 4 groups: Vit D3 group, the mixed group of Vit D3 and tamoxifen, tamoxifen group and control group. After 4 weeks, the tumor volume was measured and serum calcium and phosphorus levels were assayed. Tumor cells were analyzed with flow cytometery to detect apoptosis and cell cycle. RESULTS: Serum calcium levels in Vit D3 group were higher than those in control group(2.54±0.05 vs 2.32±0.09 mmol/L, P<0.05), but phosphorus levels were lower(1.78±0.13 vs 2.34±0.06 mmol/L, P<0.05). The apoptosis rate (%) of tumor cells in Vit D3 group was higher than that of control group (31.5±6.9 vs 7.8±2.5, P<0.05) and tumor cells were arrested in G0/G1 phase(50.0±3.2 vs 34.2±4.7, P<0.05), especially in the mixed group treated with Vit D3 and tamoxifen. CONCLUSION: Vit D3 has some synergic effect with tamoxifen and can significantly induce apoptosis of breast cancer cells and arrest tumor cells in G0/G1 phase. ......
您现在查看是摘要页,全文长 6000 字符。