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Vitamin D3对裸鼠乳腺癌生长的影响
http://www.100md.com 《第四军医大学学报》 2006年第6期
胆骨化醇,,胆骨化醇;乳腺肿瘤;细胞凋亡;细胞周期,VitaminD3对裸鼠乳腺癌生长的影响,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Effect of vitamin D3 on growth of breast cancer in nude mouse xenograft models

    ZHOU Wei, WANG JiaLin, ZHAO YaPing, WANG Yu, ZHANG Yong

    Department of Endocrinology, PLA 82nd Hospital, Huaian 223001, China

    【Abstract】 AIM: To study the effect of Vit D3 on breast cancer in nude mouse xenograft models. METHODS: Each Balb/c nude mouse was injected subcutaneously with human breast cancer cell line MCF7. Two thousand units of Vit D3 was injected in mice thigh muscle every other day and mice were fed with tamoxifen (5 mg/kg) every day. Forty experimental mice were randomly divided into 4 groups: Vit D3 group, the mixed group of Vit D3 and tamoxifen, tamoxifen group and control group. After 4 weeks, the tumor volume was measured and serum calcium and phosphorus levels were assayed. Tumor cells were analyzed with flow cytometery to detect apoptosis and cell cycle. RESULTS: Serum calcium levels in Vit D3 group were higher than those in control group(2.54±0.05 vs 2.32±0.09 mmol/L, P<0.05), but phosphorus levels were lower(1.78±0.13 vs 2.34±0.06 mmol/L, P<0.05). The apoptosis rate (%) of tumor cells in Vit D3 group was higher than that of control group (31.5±6.9 vs 7.8±2.5, P<0.05) and tumor cells were arrested in G0/G1 phase(50.0±3.2 vs 34.2±4.7, P<0.05), especially in the mixed group treated with Vit D3 and tamoxifen. CONCLUSION: Vit D3 has some synergic effect with tamoxifen and can significantly induce apoptosis of breast cancer cells and arrest tumor cells in G0/G1 phase. ......

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