更年期_____更年期；脾细胞；凋亡；Th1；Th2

更年期大鼠Th1/Th2平衡与脾细胞凋亡

http://www.100md.com 《西安交通大学学报》 2006年第1期

更年期,,更年期；脾细胞；凋亡；Th1；Th2,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的了解更年期大鼠脾细胞凋亡与辅助性T细胞1(Thelper l, Th1)和辅助性T细胞2(Thelper 2, Th2)型细胞因子的增龄性改变。方法应用Annexin VFITC/PI双染色流式细胞仪测定脾细胞凋亡率； ELISA法测血清γ干扰素(interferonγ, IFNγ)、白介素4(interleukin4, IL4)水平。结果更年期组大鼠脾细胞凋亡率(10.3% vs. 7.7%, P<0.05)、血清IFNγ质量浓度明显高于年轻组大鼠(23.80ng/L vs. 10.92ng/L，P<0.01)；更年期组大鼠与年轻组相比血清IL4质量浓度有下降趋势，但差别无显著意义(0.1296ng/L vs. 0.3094ng/L, P>0.05)；更年期组血清IFNγ/IL4比值明显高于年轻组(183.64 vs. 35.29，P<0.01)，Th1/Th2平衡朝Th1漂移。结论大鼠衰老过程中Th1/Th2平衡朝Th1漂移，脾细胞凋亡增加与IFNγ水平增高有关。

    关键词：更年期；脾细胞；凋亡；Th1；Th2

    The changes of Th1/Th2 balance and apoptosis in splenocyte of menopausal rats

    Jiang Jing, Zhang Shaofen, Zhou Shien, Xia Xian, Chen Xun, Liu Ruizi

    (Department of Gynecology, Obstetrics and Gynecological Hospital, Fudan University,Shanghai 200011;Department of Immunology, Shanghai Medical College, Fudan University, Shanghai 200032, China)

    ABSTRACT: Objective To investigate the relation between the apoptosis in splenocyte and the changes of Th1/Th2 balance in serum of menopausal rats. Methods Twenty female SD rats were divided into two groups: menopausal group(n=10;15 months), young group (n=10; 5.5 months). The apoptosis in splenocyte of rats was measured by Annexin VFITC/PI flow cytometric assay. The levels of IFNγ and IL4 in serum were measured by ELISA. Results The percentages of apoptosis cells in splenocyte of menopausal rats were significantly higher than that of young rats. The serum IFNγ level of menopausal rats was significantly higher than that of young rats(23.80ng/L vs. 10.92ng/L, P<0.01). The level of IL4 was similar in menopausal and young groups. Conclusion The increasing of apoptosis in splenocyte of menopausal rats is related to the increasing of IFNγ levels with aging. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/12/96/61/02.htm

您现在查看是摘要页，全文长 8801 字符。