黑色素瘤__(期刊论文)

对小鼠黑色素瘤细胞肺转移的抑制作用mGMCSF基因转染

http://www.100md.com 《第四军医大学学报》 2006年第6期

黑色素瘤,,黑色素瘤；小鼠；粒细胞巨噬细胞-集落刺激因子；肺肿瘤,继发性；癌症疫苗,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Inhibition of lung metastasis of B16 melanoma cells transfected with murine granulocytemacrophage colonystimulating factor in mice

    TIAN Rong, CHENG ShaoHui, YU JiYun, LI Wei, LIU YuFeng

    1Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China, 2Institute of Basic Medicine, Military Academy of Medical Sciences, Beijing 100850, China

    【Abstract】AIM: To investigate the inhibition of lung metastasis of melanoma by a tumor vaccine of B16 melanoma cells transfected with murine granulocytemacrophage colonystimulating factor (mGMCSF). METHODS: The murine melanoma cell line B16 was transfected with the recombinant plasmid containing mGMCSF. A cell line stably expressing mGMCSF was obtained by G418 selection. The Balb/c mice were administered i.v. with B16 cells transfected with or without mGMCSF respectively and the lung metastasis was examined 2 weeks later. RESULTS: The recombinant cell line B16TR was established after mGMCSF tranfection and the expression of mGMCSF was confirmed by RTPCR. The number of lung metastatic lesions of mice treated with B16TR was significantly fewer than that of mice treated with B16 (n=5, 125.8±34.3 vs 27.8±24.0, P<0.01). CONCLUSION: The mGMCSF transfection can significantly suppress the lung metastasis of melanoma in mice. The GMCSF can be a promising adjuvant in the immunotherapy of melanoma. ......

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