重组人__(期刊论文)

重组人PD1IgV融合蛋白的克隆、表达、纯化及生物学活性检测

http://www.100md.com 《第四军医大学学报》 2006年第5期

重组人,,PD1IgV；重组融合蛋白；纯化；复性；pQE30载体,0引言,1材料和方法,2结果,3讨论,【参考文献】

     cnCloning, expression, purification and biologic characteristics of rhPD1IgV protein

    WANG WeiHua, ZHANG YingQi, YAN Zhen

    Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To obtain biologically active recombinant human PD1IgV (rhPD1IgV) protein. METHODS: Gene encoding for rhPD1IgV was synthesized and cloned into the expression vector pQE30. The expression plasmid was then transformed into E. coli DH5α. The expression of the fusion protein rhPD1IgV was induced with IPTG and detected by SDSPAGE and Western blot. The biological activity of rhPD1IgV was identified by ELISA and flow cytometry. RESULTS: SDSPAGE showed that the molecular weight of rhPD1IgV was about 15×103, which was consistent with the theoretical value calculated based on the composition of amino acid. Purified rhPD1IgV was refolded, concentrated and associated well with B7H1. CONCLUSION: rhPD1IgV fusion protein is successfully cloned, expressed and purified. The protein associated with B7H1 has a strong binding activity. ......

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