雌二醇_____雌二醇；受体，阿片样，κ；CNE2细胞

17β雌二醇对CNE2细胞κ阿片受体表达的抑制作用

http://www.100md.com 《第四军医大学学报》 2006年第6期

雌二醇,,雌二醇；受体，阿片样，κ；CNE2细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】

     βestradiol suppresses expression of κOR gene in CNE2 cell line

    WEI GengZe, ZHU YunLong, HU YuZhen, ZHOU JingJun

    Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To observe the regulatory effect of 17βestradiol on the expression of kappa opioid receptor (κOR) mRNA and protein. METHODS: Semiquantitative RTPCR and Western blot were used to analyze the mRNA content and protein expression in CNE2 cells 24-72 h after treated with 17 βestradiol. RESULTS: Compared with that in control group, κOR mRNA reduced to (49.2±7.66)% (P<0.01) and (20.8±7.71)% (P<0.01) respectively, after 1 μmol/L 17βestradiol treatment for 24 and 72 h. The quantity of protein decreased to (60.8±8.36)%, (32.9±7.13)% and (29.8±8.41)% (P<0.01) respectively following 1 μmol/L 17 βestradiol treatment for 24, 48 and 72 h. CONCLUSION: Estrogen can inhibit the transcription of κOR in CNE2 cells.

    【Keywords】 estradiol; receptors, opioid, kappa; CNE2 cells

    【摘要】目的：观察雌激素对κ阿片肽受体(κOR)mRNA含量和蛋白表达的调节作用. 方法：采用半定量RTPCR和Western blot方法 ......

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