槲皮素对结肠癌HT-29细胞增殖及周期的影响
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严兴耘, 彭俊华, 张华欣, 张 峰, 卞晓红,
细胞凋亡;结肠癌;槲皮素;Caspase-3;bcl-2;bax严兴耘,彭俊华,张华欣,张峰,卞晓红,王海忠,赵涛.槲皮素对结肠癌HT-29细胞增殖及周期的影响.世界华人消化杂志2006;14(11)
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严兴耘, 赵涛, 兰州石油化工公司兰炼医院消化内科 甘肃省兰州市 730060
彭俊华, 张华欣, 张峰, 兰州军医兰州总医院检验科 甘肃省兰州市 730050
卞晓红, 兰州军医兰州总医院消化科 甘肃省兰州市 730050
王海忠, 兰州军医兰州总医院肿瘤科 甘肃省兰州市 730050严兴耘, 1990年重庆市第三军医大学学士, 主治医师, 主要从事消化系统与肿瘤研究.
通讯作者: 彭俊华, 730050, 甘肃省兰州市, 兰州军医兰州总医院检验科. junhua_p@sohu.com
电话: 0931-8975629
收稿日期: 2005-12-29 接受日期: 2006-01-13
Effects of quercetin on proliferation and cell cycle of colon carcinoma cell Line HT-29
Xin-Yun Yan, Jun-Hua Peng, Hua-Xin Zhang, Feng Zhang, Xiao-Hong Bian, Hai-Zhong Wang, Tao Zhao
Xin-Yun Yan, Zhao Tao, Department of Gastroenterology, Lanlian Hospital, Lanzhou Petrochemical Industry Corporation, LanZhou 730060, Gansu Province, China
Jun-Hua Peng, Hua-Xin Zhang, Feng-Zhang, Department of Clinical Laboratory, Lanzhou General Hospital, Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China
Xiao-Hong Bian, Department of Digestive Diseases, Lanzhou General Hospital, Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China
Hai-Zhong Wang, Tumor Department of Lanzhou General Hospital, Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China
Correspondence to: Jun-Hua Peng, Department of Clinical Laboratory, Lanzhou General Hospital, Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China. junhua_p@sohu.com
Received: 2005-12-29 Accepted: 2006-01-13
AbstractAIM:To investigate the effect of quercetin on the proliferation and apoptosis of colon carcinoma cell line HT-29 and its molecular mechanism.
METHODS: HT-29 cells were treated with different concentrations of quercetin (40×10-6, 80×10-6 and 160×10-6 mol/L) for 72 h. HT-29 cells treated with DMSO vehicle were used as the controls. MTT assay, flow cytometry, immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR) was used to observe the changes of cell proliferation, cell cycle, Caspase-3 protein expression, and bax, bcl-2, and Caspase mRNA expression in HT-29 cells, respectively ......
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