转化生长因子β1启动子区C-509T位点基因多态性与肝纤维化关系
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黄玉波, 魏红山, 董庆鸣, 刘志英, 郭晶晶,
多聚酶链式反应;限制性片段长度多态性分析;纤维化;转化生长因子β1黄玉波,魏红山,董庆鸣,刘志英,郭晶晶,成军,毛羽.转化生长因子β1启动子区C-509T位点基因多态性与肝纤维化关系. 世
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黄玉波, 北京地坛医院肝炎一科 北京市 100011
魏红山, 董庆鸣, 刘志英, 郭晶晶, 成军, 毛羽, 北京地坛医院传染病研究所 北京市 100011北京市优秀人才培养专项经费资助课题, No. 20041D0301548
通讯作者: 魏红山, 100011, 北京市, 北京地坛医院传染病研究所. drliver@163.com
电话: 010-64211031-2359
收稿日期: 2006-01-25 接受日期: 2006-02-13
Polymorphism of C-509T at promoter region of transforming growth factor β1 gene might be related to liver fibrosis
Yu-Bo Huang, Hong-Shan Wei, Qing-Ming Dong, Zhi-Ying Liu, Jing-Jing Guo, Jun Cheng, Yu Mao
Yu-Bo Huang, the First Department of Hepatitis, Beijing Ditan Hospital, Beijing 100011, China
Hong-Shan Wei, Qing-Ming Dong, Zhi-Ying Liu, Jing-Jing Guo, Jun Cheng, Yu Mao, Institute of Infectious Diseases, Beijing Ditan Hospital, Beijing 100011, China
Supported by the Fund for Foster Excellent Talents of Beijing, No. 20041D0301548
Correspondence to: Hong-Shan Wei, Institute of Infectious Diseases, Beijing Ditan Hospital, Beijing 100011, China. drliver@163.com
Received: 2006-01-25 Accepted: 2006-02-13
AbstractAIM: To establish a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect the polymorphism of C-509T at the promoter region of transforming growth factor (TGF) β1 gene and explore its probable role in liver fibrosis.
METHODS: Patients with chronic hepatitis B (n = 50) and liver cirrhosis (n = 46) were involved in this study. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was established. A set of primers was designed according to the promoter region of TGF-b1 gene. Around C-509T was the enzyme digestion site for restriction endonuclease Bsu36Ⅰ. C or T was assured based on the enzymatic result of the polymerase chain reaction (PCR) products.
RESULTS: Sequencing revealed 99% homogeneity between the two homozygous strains and the promoter region of TGF-b1 gene. The anticipated mutations appeared at position C-509T ......
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