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高效液相色谱法测定降脂减肥颗粒中葛根素的含量
http://www.100md.com 《时珍国医国药》 2006年第2期
高效液相色谱法,,降脂减肥颗粒;,葛根素;,高效液相色谱法,1仪器与试药,2实验方法,3实验结果,4讨论,参考文献:
     摘要:目的建立降脂减肥颗粒中葛根素的含量测定方法。方法采用Shim-pack(岛津)ODS C柱(150 mm × 4.6 mm,5 μm),流动相为甲醇0.1%磷酸(25.3∶74.7),流速为0.9 mg/ml,检测波长为250 nm。结果葛根素在0.114 0~0.570 0范围内线性关系良好,r=0.999 6,平均回收率为98.7%,RSD为0.67%。结论此测定方法准确,重现性好,可作为制剂的质量控制方法。

    关键词:降脂减肥颗粒; 葛根素; 高效液相色谱法

    Determination of Puerarin in Jiangzhijianfei Granule by HPLC

    LIN Shaohua1, HE Yufang2, NAN Minlun2, WANG Tiezhu2, ZHAO Quancheng2

    (1.Base Construction Office of Modern Traditional Chinese Drug & Bio-pharmacy of Jilin Province, Changchun 130041,China; 2.Jilin Natural Medicine and Technology Co.,Ltd., Changchun 130021,China)

    Abstract:ObjectiveTo establish the HPLC method for the determination of puerarin in Jiangzhijianfei granule.MethodsThe chromatographic conditions included:The column is Shim-pack ODS C18 column (150 mm×4.6 mm, 5 μm),the mobile phase is methol- water H3PO4 ,the detection wavelength is 250 nm and the column temperature is room temperature.ResultsThe linear range of puerarin was 0.114 0~0.570 0 μg, r=0.999 6.The average recovery of puerarin was 98.7% with RSD 0.67%. ConclusionThe mothod is accurate and can be used as a determination of puerarin in Jiangzhijianfei granule. ......

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