高效液相色谱法测定跌打活络微乳中蛇床子素的含量
高效液相色谱,,跌打活络微乳；高效液相色谱法；蛇床子素,1仪器与试药,2方法与结果,3讨论,参考文献：
摘要：目的建立跌打活络微乳中蛇床子素含量的测定方法。方法采用高效液相色谱法，色谱柱：Diamonsil钻石C18(5 μm，250 mm×4.6 mm)；流动相：甲醇水(80∶20)，检测波长：322 nm；流速：1 ml/min。结果蛇床子素在0.042~ 0.336 μg线性关系良好，r=0.999 7，平均回收率为100.91%，RSD为2.42%(n=6)。结论该法操作简便，结果准确，灵敏度高，重现性好，可用于该制剂的质量控制。关键词：跌打活络微乳；高效液相色谱法；蛇床子素
Determination of Osthole in Dieda Huoluo Microemulsions by HPLC
WANG Lisheng， ZHAN Jiankun， ZHOU Liling
(1.College of Traditional Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China; 2.Grade 2002 Undergraduate of Pharmaceutical Engineering, College of Traditional Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China)
Abstract：ObjectiveTo establish the determination method for osthole in Dieda Huoluo Microemulsions. MethodsHPLC was used with DiamonsilC18 column (250 mm×4.6 mm, 5 μm), detection wavelength at 322 nm, mobile phase: methanol-water (80∶20), flow rate: 1ml/min. ResultsOsthole has good linear relationship between 0.042~0.336 μg, r=0.999 7, the recovery of osthole was 100.91% and RSD was 2.42% (n=6). ConclusionThe method is simple, sensitive and reliable to carry out to control the quality of this preparation. ......
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