缺氧__(期刊论文)

缺氧诱导滋养细胞转化生长因子3β表达与调控

http://www.100md.com 《第四军医大学学报》 2005年第4期

缺氧,,缺氧；滋养层；缺氧诱导因子1；转化生长因子3β,1材料和方法,2结果,3讨论,【参考文献】

     Effects of hypoxia on transforming growth factor3β expression and its regulation in trophoblast

    WU WeiGuang1,YAO YuanQing1,LI DongHong1,YU YueCheng2,FAN XiaoBin1

    1Department of Obstetrics ＆ Gynecology,Tangdu Hospital,Xian 710038,China,2Department of Obstetrics ＆ Gynecology,Xijing Hospital,Fourth Military Medical University,Xian 710033,China

    【Abstract】 AIM: To investigate the effect of hypoxia on transforming growth factor3β(TGF3β) expression in trophoblasts and its regulation by hypoxiainducible factor1 (HIF1). METHODS: The BeWo cells,derived from trophoblasts,were divided into four groups to be cultured: control group,hypoxic group,antisense group and sense group.The cells in antisense group and sense group were incubated under hypoxic condition after cultured with HIF1α oligonucleotide under normal conditions for 6 h.After 48 h,the cells were harvested.The mRNA expression of HIF1α and TGF3β was evaluated by real time PCR and the protein expression of HIF1α and TGF3β was measured by Western blot.RESULTS: Compared with those in the control group,both the mRNA expression and the protein expression of HIF1α and TGF3β increased in hypoxic group.Compared with the sense group,the mRNA expression and the protein expression of HIF1α and TGF3β decreased in antisense group.CONCLUSION: The hypoxia can induce the expression of TGF3β in trophoblasts and the inducible effect of hypoxia on TGF3β expression may be mediated by HIF1. ......

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