肝肿瘤__(期刊论文)

耐长春新碱肝癌细胞系HCC7721/VCR的建立

http://www.100md.com 《第四军医大学学报》 2005年第6期

肝肿瘤,,抗药性，多药；肿瘤细胞，培养的；肝肿瘤；长春新碱,1材料和方法,2结果,3讨论,【参考文献】

     Establishment of human multidrugresistant hepatocellular carcinoma cell line HCC7721/VCR

    ZHANG XiDong1，GONG AiChun1，JING XiaoWei2，HAN QuanLi2

    1Department of Hepatobiliary Surgery，2Department of Geratology，General Hospital of Air Forces PLA，Beijing 100036，China

    【Abstract】 AIM: To establish human multidrugresistant hepatocellular carcinoma cell line HCC7721/VCR and to study its biological characteristics.METHODS: Using intermittent administration of highdose VCR,human hepatocellular carcinoma cell line HCC7721 was induced to human multidrugresistant hepatocellular carcinoma cell line HCC7721/VCR.In vitro drug sensitivity of HCC7721/VCR and HCC7721 was determined by MTT assay,and the distribution of their cell cycles was assayed by flow cytometry.RESULTS: ① HCC7721/VCR was resistant to many chemotherapy agents.The IC50 value of HCC7721/VCR to VCR was 415 times higher than that of HCC7721,and its IC50 to other chemotherapy drugs was 2~4 times higher than that of HCC7721.② The multiplication time of HCC7721/VCR prolonged and the number of cells in Sphase decreased while that in G1phase and G2phase increased.CONCLUSION: ① We successfully established a multidrugresistant cell line HCC7721/VCR,which has the basic characteristics of MDR cells.② Compared with that of its parent cell line HCC7721,the IC50 value of HCC7721/VCR to VCR increases 415 times and its distribution of cell cycle changes. ......

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