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编号:10969233
血管紧张素Ⅱ对人心房肌细胞内Ca2+的影响及替米沙坦的拮抗作用
http://www.100md.com 《第四军医大学学报》 2005年第5期
心房肌细胞,,心房肌细胞;血管紧张素Ⅱ;钙;替米沙坦,1材料和方法,2结果,3讨论,【参考文献】
     【Abstract】 AIM: To examine the effects of angiotensin Ⅱ (AngⅡ) on intracellular free calcium concentration ([Ca2+]i) in human atrial myocytes and the antagonism effect of telmisartan. METHODS: Single human atrial myocyte was isolated. Four groups were included in the experiment: control group, AngⅡ group, telmisartan group, and AngⅡ + telmisartan group. Confocal microscope was used with Fluo3/AM as calcium indicator to detect the changes of [Ca2+]i immediately and 15 minutes respectively after the drug intervention. RESULTS: The intracellular fluoresence intensity of singular cardiomyocytes in control and telmisartan groups was low and the intracellular fluoresence intensity of singular cardiomyocytes in AngⅡ group was significantly higher than that in the control group 15 minutes after intervention (P<0.01). When telmisartan was added simultaneously with AngⅡ intervention, the values were much lower compared with those in AngⅡ group (P<0.01). CONCLUSION: AngⅡ induces intracellular calcium overload in human atrial myocytes and telmisartan can decrease [Ca2+]i overload in human atrial myocytes induced by AngⅡ. ......

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