Effects of lowª²intensity pulsed electromagnetic fields on osteoblasts in rats

    LI Liª²Rong1,LUO Erª²Ping1,SHEN Guangª²Hao1,HAN Liª²Hua2£¬WU Xiaoª²Ming1,ZHOU Yaª²Lei1£¬ZHOU Longª²Fu1

    1Department of Military Medical Equipment & Metrology,School of Biomedical Engineering,2PLA Institute of Orthopaedics,Xijing Hospital£¬Fourth Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To observe the effects of lowª²intensity pulsed electromagnetic fields (PEMFs) on the proliferation,differentiation and bone tissueª²like formation of cultured rat osteoblasts.METHODS: Osteoblasts isolated from newly born (<24 h) SD rats were cultured by explants outgrowth technique.The osteoblasts in the PEMFs group were cultured for 30 min in the PEMFs with the intension of electromagnetic fields 8 Gs,frequency 15 Hz.MTT assay,alkaline phosphatataseª²specific activity assay and tetracycline fluorescence labeling assay were used to observe the effects of PEMFs on the osteoblasts¬ð proliferation,differentiation and bone tissueª²like formation.RESULTS: ¢Ù MTT assay demonstrated that the A490 nm value of sham group and PEMFs group was respectively (0ª±330¡À0ª±020) and (0ª±379¡À0ª±038),with obvious difference(P<0ª±01).¢Ú ALP assay displayed that the A410 nm value of sham group and PEMFs group was respectively (0ª±170¡À0ª±029)and (0ª±206¡À0ª±018),with obvious difference(P<0ª±01).¢Û The calcium node in PEMFs group was far more than that in sham group.CONCLUSION: The PEMFs can accelerate the cell proliferation,upregulate the ALP level and increase the bone tissueª²like formation of rat osteoblasts in vitro. ......