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编号:10969015
正常大鼠海马NMDA受体2B亚单位蛋白及其mRNA的平行分布规律
http://www.100md.com 《徐州医学院学报》 2006年第1期
大鼠,,NMDA受体;免疫组织化学;原位杂交;海马;大鼠,1材料和方法,2结果,3讨论,参考文献:
     摘要 :目的 观察正常大鼠海马NMDA受体2B亚单位(NR2B)蛋白质及其mRNA的基础性表达特征及两者在海马不同区域的表达关系。 方法 正常SD大鼠海马,灌注取脑,连续冰冻切片,ABC免疫组织化学方法染色和原位杂交组织化学染色,光镜下观察NR2B蛋白质及其mRNA的表达。 结果 NR2B蛋白质在正常大鼠海马CA1区、CA3区及齿状回的免疫反应强度存在明显差异,CA1区最强,CA3区次之,齿状回着色较弱。NR2B mRNA在正常大鼠海马CA1区、CA3区及齿状回的原位杂交组织化学反应强度与NR2B蛋白质免疫反应强度存在平行变化,也是CA1区最强,CA3区次之,齿状回着色较弱。 结论 NR2B蛋白质及其mRNA在正常大鼠海马CA1区、CA3区及齿状回的表达强度不同,并存在平行表达差异关系,这种基础状态的分布特征可能与海马缺血易损性和耐受性相关。

    关键词 :NMDA受体;免疫组织化学;原位杂交;海马;大鼠

    The normal distribution of immunoreactive protein and mRNA of NMDA receptor 2B subunit(NR2B)in rat hippocampus

    TENG Da-cai,XUTie-jun,ZHANG Feng-zhen

    (Department of Human Anatomy and Neurobiology,Xuzhou Medical College,Xuzhou,Jiangsu221002,China)

    Abstract:Objective To study the normal morphological distribution of immunoreactive protein and mRNA of NMDA re-ceptor2B subunit(NR2B)in rat hippocampus to investigate the relation between the protein and the mRNA.Methods After transcardiac perfusion with4%paraformaldehyde solution,the brain was removed to cut serial cryo-sections,which were pro-cessed by ABC immunohistochemistry and in situ hybridization(ISH)to study the expression of NR2B protein and its mRNA re-spectively.Results NR2B and NR2B mRNAwere widely distributed in all normal hippocampal subfields.The immunoreactivi-ty of NR2B was stronger in the CA1than in the CA3subfield ......

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