Identification of interaction and exact region for interaction between Nª²terminal of MINT and Vav1

    DONG Xiao,HAN Hua

    Department of Medical Genetics & Developmental Biology,School of Basic Medicine,Fourth Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To study the interactions between the Nª²terminal of Msx2ª²interacting nuclear target protein (MINT) and Vav1 and to further clarify the transcriptional regulation of MINT and the mechanism of its function.METHODS: Yeast twoª²hybrid assay was conducted to analyze the interaction between the Nª²terminal of MINT and Vav1.The bait plasmid was constructed by cloning MINTª²Fx (X represents 1ª²6) into the vector pGBKT7,several different fragments of the Cª²terminal of Vav1 were cloned into the vector pGADT7 or pACT2 and their interaction was tested by the growth of yeast assay.RESULTS: Definite interaction was found between pACT2ª²V23# and pGBKT7ª²F1 or pACT2ª²V8900 and pGBKT7ª²F1,while no interaction was found between other clones.CONCLUSION: ¢Ù MINT can interact with Vav1 by its Nª²terminal.¢Ú The exact region for the interaction of MINT and Vav1 lies in the SH2ª²SH3ª²C terminal of Vav1,but single SH2 or SH3ª²C terminal of Vav1 can not interact with MINT. ......