Effects of circadian rhythm on hemorrheology and erythrocyte fragility in PEMFs exposed mice

    SHEN Guangª²Hao1£¬LUO Erª²Ping1£¬LU Liª²Hua2£¬WU Xiaoª²Ming1,XU Qiaoª²Ling3,ZHOU Longª²Fu1,LI Liª²Rong1£¬LIU Luª²Wei4

    1Department of Military Medical Equipment & Metrology,School of Biomedical Engineering,2Department of Chemistry,School of Pharmacy,3School of Nursing,4Department of Outpatient,Fourth Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To study the effects of circadian rhythm on the hemorrheology and erythrocyte fragility by exposing mice to pulsed electromagnetic fields (PEMFs).METHODS: Thirty male BALB/c mice were randomly divided into 3 groups: control,magnetic A,and magnetic B.The mice of both magnetic groups (A,B) were exposed to PEMFs(f=15 Hz) 6 h per day,with the intensity set to 8¡Á10-4 T,but the two magnetic groups were exposed at different time period,with daytime 10¡Ã00-16¡Ã00 for group A and nighttime 22¡Ã00-4¡Ã00 for group B.The mice of control group were fed in coils with sham exposure.After 10 d,the changes of the properties of hemorrheology and erythrocyte fragility were observed.RESULTS: Statistically significant differences were observed in the values of hemorrheology and erythrocyte fragility between the magnetic group (A or B) and control group (P<0ª±05).The values of hemorrheology and the concentrations of normal hemolysis in magnetic group A obviously decreased (P<0ª±05),compared with those of magnetic group B.But,no significant differences were found in the concentrations of complete hemolysis between the two magnetic groups (P>0ª±05).CONCLUSION: PEMFs are effective in improving the hemorrheology and decreasing the erythrocyte fragility.Effects of exposure in daytime is better than that in nighttime.The bioª²effects of PEMFs are affected by the circadian rhythm. ......