肌苷_____](论著)

肌苷对大鼠脑缺血再灌注后GAP43 mRNA表达影响

http://www.100md.com 《齐鲁医学杂志》 2006年第1期

肌苷,,],肌苷；脑缺血;再灌注；GAP43；基因表达；再生,肌苷对大鼠脑缺血再灌注后GAP43mRNA表达影响,1材料与方法,2结果,3讨论,[参考文献]

     [摘要] 目的探讨肌苷对脑缺血再灌注后中枢神经再生的作用。方法线栓法建立大脑中动脉缺血再灌注模型，随机分为治疗组和对照组，每组再随机分为缺血1.5 h再灌注2 h、12 h、1 d、2 d、3 d、7 d、14 d组(n=4)，另取4只作假手术组。应用BEDERSON等神经功能评分法评定神经功能，原位杂交技术检测脑缺血再灌注后各时间点脑组织生长相关蛋白基因(GAP43 mRNA)的表达。结果对照组于再灌注2 h~7 d、治疗组于再灌注12 h~7 d,GAP43 mRNA表达与假手术组比较明显增多(t=2.70~29.84，P<0.05)，治疗组与对照组比较GAP43 mRNA表达于再灌注2 h一过性下降，12 h和7 d明显增高(t=1.97~7.41，P<0.05);治疗组与对照组比较神经功能恢复于再灌注7 d有显著性差异(t=2.31,P<0.05)。结论肌苷可促进大鼠脑缺血再灌注后神经功能恢复，其作用机制可能是通过调节与神经轴突再生有关的GAP43 mRNA表达而实现的。

    [关键词] 肌苷；脑缺血;再灌注；GAP43；基因表达；再生

    EFFECTS OF INOSINE ON EXPRESSION OF GAP43 mRNA AFTER CEREBRAL ISCHEMIC REPERFUSION IN RATS

    LIU BOCHEN, GUO YUNLIANG

    (Department of Nuerosurgery, The Second Affiliated Hospital of Qingdao University Medical College, Qingdao 266042, China)

    [ABSTRACT]ObjectiveTo explore the effects of inosine on central nervous regeneration after cerebral ischemic reperfusion. MethodsThe model of the middle cerebral artery occlusion (MCAO) in SD rats was established by nylon monofilament suture. The rats were randomly divided into treated and control groups, and they were further divided into 2, 12hour, 1, 2, 3, 7 and 14day reperfusion subgroups. The other four rats were shamoperated. BEDERSON and other neurological grading were used to investigate the nerve functions and in situ hybridization for the expression of GAP43 mRNA in brain tissue. Results The GAP43 mRNA expressions at two hours to seven days of reperfusion in the control group and at 12 hours to seven days of reperfusion in the treated group were more significant compared with those in the shamoperated group (t=2.70-29.84 ......

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