反义Survivin核酸诱导凋亡及逆转胃癌耐药机制的实验研究
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马 荣, 陈曦海, 张岂凡, 唐丽萍
反义Survivin核酸;胃癌;凋亡;泰索帝;MDR-1;化疗敏感性,马荣,唐丽萍,陈曦海,张岂凡,通讯作者,Experimentalst
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马荣, 唐丽萍, 哈尔滨医科大学附属肿瘤医院妇科 黑龙江省哈尔滨市 150040
陈曦海, 哈尔滨医科大学附属第四医院 黑龙江省哈尔滨市 150001
张岂凡, 哈尔滨医科大学附属第二医院普外科 黑龙江省哈尔滨市 150040
马荣, 1997年东南大学本科毕业, 2003年哈尔滨医科大学硕士研究生毕业, 2006年博士研究生毕业, 主治医师, 主要从事肿瘤学研究.
黑龙江省教育厅一般项目基金资助, No. 10551152
通讯作者: 马荣, 150040, 黑龙江省哈尔滨市哈平路150号, 哈尔滨医科大学附属肿瘤医院妇科. maronghai@yahoo.com.cn
电话: 0451-86298537
收稿日期: 2006-02-06 接受日期: 2006-02-20
Experimental study on inducing apoptosis and reversing drug resistance of gastric cancer cells SGC7901 by antisense Survivin RNA
Rong Ma, Xi-Hai Chen, Qi-Fan Zhang, Li-Ping Tang
Rong Ma, Li-Ping Tang, Gynecology, the Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China
Xi-Hai Chen, Department of Surgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Qi-Fan Zhang, Department of Surgery, the Second Affiliated Hospital of Harbin Medical University Hospital, Harbin 150040, Heilongjiang Province, China
Supported by the Generic Item Foundation of Educational Department of Heilongjiang Province, No. 10551152
Correspondence to: Rong Ma, Gynecology, Harbin Medical University Affiliated Tumor Hospital, Harbin 150040, Heilongjiang Province, China. maronghai@yahoo.com.cn
Received: 2006-02-06 Accepted: 2006-02-20
Abstract
AIM: To explore the effect of antisense Survivin RNA on the apoptosis, chemosensitivity to taxotere and drug resistance of gastric cancer cells SGC7901.
METHODS: SGC7901 cells were transfected with antisense Survivin eukaryotic vector (anti-pcDNA3-svv) by lipofectamine, and the positive clones were screened out. Survivin protein and multi-drug resistance-1 (MDR-1) mRNA were determined by Western blot and reverse transcription polymerase chain reaction (RT-PCR). The apoptosis of SGC7901 cells was observed by electronic microscopy and TUNEL method, and the sensitivity of the cells to taxotere was examined by MTT assay ......
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