人巨细胞病毒先天性潜伏感染再激活致老年小鼠肝脏的损伤
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胡雪影, 王明丽
人巨细胞病毒;潜伏再激活感染;肝脏;婴儿肝炎综合症,胡雪影,王明丽,胡雪影,通讯作者,Studyonliverdamageinducedbyrea
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胡雪影, 王明丽, 安徽医科大学微生物学教研室 安徽省合肥市 230032
胡雪影, 2004级安徽医科大学硕士生.
教育部科学技术研究重点项目, No. 01052
安徽省“十五”生物医药重大科技专项, No. 01303003
通讯作者: 王明丽, 230032, 安徽省合肥市, 安徽医科大学微生物学教研室. wangmlli@mail.hf.ah.cn
电话: 0551-5123422 传真：0551-5123422
收稿日期: 2006-03-04 接受日期: 2006-03-29
Study on liver damage induced by reactivation of congenital latent infection of human cytomegalovirus in aged mice
Xue-Ying Hu, Ming-Li Wang
Xue-Ying Hu, Ming-Li Wang, Department of Microbiology, Anhui Medical University, Hefei 230032, Anhui Province, China
Supported by the Key Item of Science and Technology from Ministry of Education, No. 01052, and the Important Special Item of Biological Medicine in the Tenth Five-Year Plan of Anhui Province, No. 01303003
Correspondence to: Ming-Li Wang, Department of Microbiology, Anhui Medical University, Hefei 230032, Anhui Province, China. wangmlli@mail.hf.ah.cn
Received: 2006-03-04 Accepted: 2006-03-29
Abstract
AIM: To establish the model of liver damage induced by reactivation of congenital latent infection of human cytomegalovirus (HCMV) in aged mice, and to explore the pathogenic mechanism of HCMV reactivation.
METHODS: Firstly, the mouse model of congenital latent HCMV infection was established. After raised in specific pathogen free (SPF) barrier system for 18 mo, 9 offspring mice served as cell controls and 18 were taken out for experiment, half of which were treated with cyclophosphamide to reactivate the HCMV infection. The mice without HCMV infection were used as normal controls. All the mice were sacrificed to obtain liver tissues for virus isolation, polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR) and HE stainging.
RESULTS: HCMV was isolated from the supernatant of liver tissue homogenate only in the group of infection- reactivated mice. HCMV DNA was detected by PCR in the supernatant of cell culture with cytopathogenic effect (CPE) ......
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