Effects of different splicing mouse Era on Lª² 929 cells

    DONG Ke,CHEN Suª²Min,LI Yunª²Feng,WU Yuanª²Ming,CHEN Nanª²Chun,ZHANG Xiaoª²Nan

    Department of Biochemistry £¦ Molecular Biology,School of Basic Medicine,Fourth Military Medical University,Xi¡¯an 710033,China

    ¡¾Abstract¡¿ AIM£º To construct complete inducible mammalian expression vectors that can express two alternative splicing mouse eras and to study the effects of two alternative splicing mouse era genes on cells growth.METHODS£º Two inducible vectors that express two alternative splicing mouse Eras were constructed.The inducible vectors and pERV3 were then stably coª²transfected into Lª²929 cells.After transfected cells were induced by PonA,the protein expression was detected by Western Blotting,the proliferation of cells was measured by MTT and the cell cycles were observed by flow cytometry.RESULTS£º Two kinds of cell lines that can express different splicing mouse Era proteins were obtained.The growth of cells was accelerated when the cells were induced by PonA and the percentages of G2/M phase cells decreased.CONCLUSION£º Two alternative splicing genes play important roles in the eukaryote cell cycle. ......