环氧合酶-2对人肝癌细胞增殖和凋亡的调节作用
http://www.100md.com
杨 震, 秦成勇, 朱菊人, 任万华, 王 旻
环氧合酶-2;肝癌;塞来昔布;Survivin;多药耐药杨震,秦成勇,朱菊人,任万华,王旻.环氧合酶-2对人肝癌细胞增殖和凋亡的调节作用. 世界华人消化杂志2006;14(14)1382-
 |
| 第3页 |
 |
| 第1页 |
参见附件(1045KB，6页)。
杨震, 朱菊人, 任万华, 山东省立医院肝病中心 山东省济南市 250021
秦成勇, 山东省立医院消化科 山东省济南市 250021
王旻, 山东省立医院肿瘤中心 山东省济南市 250021
杨震, 2004年山东大学临床医学院博士, 主治医师, 主要从事肝病的治疗.
通讯作者: 朱菊人, 250021, 山东省济南市经五路324号, 山东省立医院肝病中心. zhujrchina@hotmail.com
传真: 0531-86025677
收稿日期: 2006-03-03 接受日期: 2006-04-06
Modulatory roles of cyclooxygenase-2 in proliferation and apoptosis of hepatocarcinoma cells
Zhen Yang, Cheng-Yong Qin, Ju-Ren Zhu, Wan-Hua Ren, Min Wang
Zhen Yang, Ju-Ren Zhu, Wan-Hua Ren, Center of Hepatopathy, Shandong Provincial Hospital, Ji’nan 250021, Shandong Province, China
Cheng-Yong Qin, Department of Digestive Diseases, Shandong Provincial Hospital, Ji’nan 250021, Shandong Province, China
Min Wang, Center of Oncology, Shandong Provincial Hospital, Ji’nan 250021, Shandong Province, China
Correspondence to: Dr. Ju-Ren Zhu, Center of Hepatopathy, Shandong Provincial Hospital, 324 Jingwu Road, Ji’nan 250021, Shandong Province, China. zhujrchina@hotmail.com
Received: 2006-03-03 Accepted: 2006-04-06
Abstract
AIM: To observe the expression of cyclooxy-genase-2 (COX-2) and investigate the effects of COX-2 inhibitor, celecoxib, on the proliferation and apoptosis of hepatocarcinoma cells.
METHODS: The expression of COX-2 was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). The effect of celecoxib on the growth of hepatocarcinoma cells (HepG2 and Bel-7402) were evaluated by MTT assay and changes proliferation cell nuclear antigen (PCNA). The apoptosis of hepatocarcinoma cells induced by celecoxib and P-gp expression were detected by electron microscopy and flow cytometry. The expression of Survivin was analyzed by RT-PCR.
RESULTS: Celecoxib inhibited the growth and induced apoptosis of hepatocarcinoma cells in a time- and concentration-dependent manner ......
您现在查看是摘要介绍页，详见PDF附件(1045KB，6页)。