摘要：目的：研究不明原因反复体外受精—胚胎移植(试管婴儿，IVF-ET)失败的患者机体T辅助细胞1(Th1)及T辅助细胞2(Th2)免疫应答方式，为治疗不明原因反复体外受精-胚胎移植失败提供理论依据。方法：将不明原因的三次及三次以上IVF-ET失败的患者作为研究对象，一个周期IVF-ET成功妊娠的患者作为对照组，抽取非治疗周期静脉血分离单个核细胞并于37℃，5%CO2培养箱中培养72h，分离上清，ELISA法测定上清液中INF-γ/IL10含量，计算INF-γ/IL10的比值，从而反映机体Th1、Th2细胞的免疫应答方式。结果：反复IVF-ET失败的患者9例中有7例TH1细胞因子(INF-γ)浓度是Th2细胞因子(IL10)的3倍，有显著性差异；反复IVF-ET失败的患者9例中有4例机体Th1细胞因子(INF-γ)是Th2细胞因子(IL10)的9倍，呈Th1强势反应，而对照组9例中仅1例且发生早期流产，有统计学意义。结论：部分反复体外受精-胚胎移植失败的患者机体Th1 Th2免疫应答失衡，呈Th1优势反应，提示细胞免疫过强。

    关键词： 反复体外受精-胚胎移植失败； T辅助细胞； 优势反应

    Study on T Helper Cell Immune Response Type in Women with Unexplained Recurrent IVF-ET Failure

    YANG Gui-yan, JIANG Ying-hua, WU Hong-qiu, et al

    (Maternal and Children Health Care Hospital of Zhuhai, Guangdong Zhuhai 519000,China)

    Abstract： Objective：To study the Th1 and Th2 response type in women with unexplained recurrent IVF-ET failure, and to provide theoretic evidence on dealing with recurrent IVF-ET failure. Method： Blood sample was taken from 9 women received 3 unexplained failure IVF-ET cycles (study group), while 9 women with successful IVF-ET cycle being controlled. The lymphocyte was separated, and then was cultured at 37℃ ......