三氧化二砷_____]_三氧化二砷;

应用DNA微阵列技术研究三氧化二砷对RPMI 8226细胞的作用

http://www.100md.com 《中西医结合学报》 2006年第2期

三氧化二砷,,],三氧化二砷;,DNA微阵列;,多发性骨髓瘤,应用DNA微阵列技术研究三氧化二砷对RPMI8226细胞的作用,1材料与方法,2结果,3讨论,[参考文献]

     [摘要] 目的：应用cDNA芯片技术研究三氧化二砷对多发性骨髓瘤细胞株RPMI 8226的作用。方法：采用包含4 096个人类基因的cDNA表达谱芯片，检测三氧化二砷作用于RPMI 8226细胞24 h前后其基因表达的变化。结果：在mRNA水平上，273个基因的表达发生了明显改变，其中121个基因表达上调，152个基因表达下调。结论：三氧化二砷可引起RPMI 8226细胞株一系列基因表达的改变。ZFYVE16、TXNIP及ALK1基因可能与RPMI 8226细胞的分化与凋亡密切相关。

    [关键词] 三氧化二砷; DNA微阵列; 多发性骨髓瘤

    Changes of gene expression profile of multiple myeloma cell line RPMI 8226 treated by arsenic trioxide

    MengChang WANG, ShanXi LIU, PengBo LIU

    (Department of Hematology, First Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30022, USA)

    ABSTRACT Objective: To compare the changes of gene expression profiles of multiple myeloma cell line RPMI 8226 before and after 24hour intervention of arsenic trioxide. Methods: The responses of the RPMI 8226 cells to arsenic trioxide were determined with cDNA microarray which included 4 096 different human genes. Results: Of these 4 096 genes, the expressions of 273 genes were altered significantly at mRNA level. The expressions of 121 genes were upregulated while the expressions of 152 genes were downregulated. Conclusion: The effect of arsenic trioxide on RPMI 8226 cells is related to changing the expression levels of a number of genes. ZFYVE16, ALK1 and TXNIP genes may play important roles in apoptosis and differentiation of RPMI 8226 cells. ......

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