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硫辛酸对细胞DNA损伤保护作用的初步研究
http://www.100md.com 《第四军医大学学报》 2006年第8期
过氧化氢,,过氧化氢;DNA;慧星试验;硫辛酸,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Preliminary investigation on protective function of lipoic acid from DNA damage

    CHEN HongLi, HAI ChunXu, LIANG Xin, ZHANG XiaoDi, LIU Rui

    Department of Toxicology, School of Preventive Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To the study doseeffect relationship between the DNA damage of HepG2 cells and the effect of different concentrations of hydrogen peroxide (H2O2) for 2 h respectively, and to observe the protective function of lipoic acid from the DNA damage of cells at the same time. METHODS: HepG2 cells were divided into control group, damage group and protective group, then were added with different concentrations of H2O2 and lipoic acid. Two hours later, the single cell gel electrophoresis (SCGE) was used to detect the DNA damage. RESULTS: The DNA damage of cells was more serious with H2O2 concentration increasing. The tail length, the tail DNA content and the tail moment significantly increased (P<0.05). Lipoic acid could significantly decrease the tail length, the tail DNA content and the tail moment of damaged cells (P<0.05). CONCLUSION: H2O2 causes DNA damage of cells in a doseeffect manner, while lipoic acid can reduce effectively this DNA damage caused by H2O2. ......

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