高效液相色谱法测定人血浆中全反式维甲酸血药浓度
血药浓度,,全反式维甲酸；血药浓度；高效液相色谱法,1材料与方法,2结果,3讨论,参考文献:
摘要:目的 建立高效液相色谱法测定人血浆中全反式维甲酸浓度。方法 色谱柱为Supelcosil LC18DB柱(5 μm,4.6 mm×250 mm)；流动相为甲醇水冰乙酸(体积比82∶17∶1)；柱温为室温；流速1.5 mL·min-1；检测波长为340 nm。结果 全反式维甲酸血药质量浓度在10～1000 ng·mL-1范围内，与峰面积比有良好的线性关系(r=0.9997)，最低检测浓度为2.5 ng·mL-1。平均方法回收率为(94.5±4.7)%(n=15)。日内RSD≤4.1%，日间RSD≤6.2%。结论 本方法简单、快速、灵敏、重现性好，适用于全反式维甲酸临床血药浓度监测及人体药代动力学研究。关键词:全反式维甲酸；血药浓度；高效液相色谱法
Determination of alltrans retinoic acid in human plasma with HPLC
WANG Xiaoping,REN Bin
(1. Department of Pharmacy,Haizhu Red Cross Hospital,Guangzhou 510250,China; 2.Department
of Pharmacy,The First Affiliated Hospital of Sun YatSen University,Guangzhou 510080,China)
Abstract: Objective To establish a HPLC method for determination of alltrans retinoic acid in human plasma. Methods A Supelcosil LC18DB column (5 μm,4.6 mm×250 mm) was adopted. Mobile phase was a mixture of methanol,water and glacial acetic acid(82∶17∶1). The flow rate was 1.5 mL·min-1. The UV detection wave was 340 nm. ResultsThe assay procedure was shown to produce linear calibration curves over the range of 10 ng·mL-1 to 1000 ng·mL-1 of alltrans retinoic acid in plasma (r=0.9997). Within the range,the recovery rate was (94.5±4.7)%,and the intraday RSD and interday was less than 4.1% and 6.2%,respectively. The detection limit of all trans retinoic acid was 2.5 ng·mL-1. Conclusions This method is repeatable,convenient and sensitive for plasma concentration monitoring and clinical pharmacokinetics study of alltrans retinoic acid. ......
您现在查看是摘要页，全文长 6276 字符。