微生物比浊法_____微生物比浊法；美帕曲星；益列康宁片

微生物比浊法测定益列康宁片中美帕曲星的含量

http://www.100md.com 《广东药学院学报》 2004年第2期

微生物比浊法,,微生物比浊法；美帕曲星；益列康宁片,1仪器与试药,2方法与结果,3讨论

     摘要目的：建立益列康宁片中美帕曲星含量的测定方法。方法：用微生物比浊法，采用啤酒酵母菌为试验菌，高、低浓度剂间比为1∶1.56，试管尺寸为20 mm×150 mm，培养温度为35～37 ℃，培养时间为5 h。结果：美帕曲星浓度在0.02～0.2 U/mL的范围内，浓度的对数与吸收度成良好的线性关系，r=0.9973。平均回收率为100.1%，RSD(n=9)=0.93%,平均可信限率为1.9% 。结论：本法简便，快速，结果准确，可用于该药的含量测定。

    关键词微生物比浊法；美帕曲星；益列康宁片

    Determination of mepartricin in ipertrofan tablets by microbiological turbidimetry

    HE Liming, ZHANG Huiwen, HUANG Xiaobi

    (Guangzhou Institute for Drug Control, Guangzhou, Guangdong 510160)

    Abstract Objective : To establish a method for the determination of mepartricin in ipertrofan tablets . Methods: Using Saccharomyces cerevisiae as the test organism, the ratio of the high dose and the low dose was 1∶1.56, the tube volume was 20 mm×150 mm. Incubation temperature was maintained at 35～37 ℃，incubate the tubes for 5 hours. Results: The calibration curve was linear with the logarithm of concentration range of 0.02～0.2 U/mL of Mepartricin with r=0.9973. The average recovery rate was 100.1%, RSD was 0.93%, and the average confidencelimit rate was 1.9%. Conclusions: The method is simple, rapid, accurate, and may be applied in the assaying of the drug. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/11/87.htm

您现在查看是摘要页，全文长 8180 字符。