海南省恶性疟原虫cg2基因ω重复区多态性与氯喹抗性关系分析
恶性疟原虫,,恶性疟原虫；氯喹抗性；cg2基因多态性；套式PCR,1材料与方法,2结果,3讨论
摘 要 目的 了解海南省恶性疟原虫cg2基因ω重复区多态性与氯喹抗性的关系。方法 采用套式PCR技术特异扩增恶性疟原虫海南分离株cg2基因ω重复区，并对部分目的基因片段进行直接测序和序列分析。结果 氯喹抗性株和敏感株均可扩增得到cg2基因ω重复区，大小约在180 bp至550 bp之间。结论 海南省恶性疟原虫cg2基因ω重复区多态性与氯喹抗性没有明显关系。关键词 恶性疟原虫；氯喹抗性；cg2基因多态性；套式PCR
Polymorphism analysis of the cg2 gene ω repeat region and the relationship with chloroquine resistance of Plasmodium falciparum isolates from Hainan Province
LI Xiaobo，JIANG Gangfeng，CHEN Peiquan
(1.Department of Medical Parasitology，Guangdong College of Pharmacy，Guangzhou,Guangdong 510240；2.Institute of Tropical Medicine，Guangzhou University of Traditional Chinese Medicine，Guangzhou,Guangdong 510405)
Abstract Objective To study the correlation between the Chloroquine resistance and cg2 gene ω repeat region of Plasmodium falciparum isolates from Hainan Province. Methods Nested PCR technique was applied to amplify the cg2 gene ω repeat region of P.falciparum isolates from Hainan Province. Some PCR products were directly sequenced for the polymorphism analysis. Results Both Chloroquine resistant(CQR)and Chloroquine sensitive(CQS) isolates produced cg2 gene ω repeat region products with the sizes from 180bp to 550bp. Conclusion There is no obvious correlation between the cg2 gene ω repeat region polymorphism and Chloroquine resistance of P.falciparum isolates from Hainan Province. ......
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