肝肿瘤细胞_____环氧合酶2；血管内皮生长因子；肝癌

肝肿瘤细胞中环氧合酶2与血管内皮生长因子表达的关系

http://www.100md.com 《广东药学院学报》 2004年第2期

肝肿瘤细胞,,环氧合酶2；血管内皮生长因子；肝癌,1材料与方法,2结果,3讨论

     摘要目的：探讨肝肿瘤细胞环氧合酶2(COX2)和血管内皮生长因子(VEGF)关系。方法：培养肝癌细胞株(BEL7402)提取mRNA, 用ELISA方法检测细胞培养基中VEGF水平，用RTPCR分别检测COX2和VEGF的mRNA。结果：正常培养的肝癌细胞能同时检测到COX2和VEGF的mRNA；COX2抑制剂赛来昔布(celecoxib,商品名：西乐葆)能抑制VEGF的表达；COX2在mRNA水平调控VEGF的表达。结论：COX2在VEGF的分泌与合成中起重要作用，从而影响肿瘤新生血管生成及肿瘤生长。

    关键词环氧合酶2；血管内皮生长因子；肝癌

    Correlation of cyclooxygenase2 with the expression of vascular endothelial growth factor in hepatocellular carcinoma cell lines BEL7402

    PAN Deshun,CHEN Weiqiang, WU Peicheng

    (1. Section of Biopharmaceutics, Guangdong College of Pharmacy, Guangzhou, Guangdong 510240; 2. Section of Pathological Physiology, Guangdong College of Pharmacy, Guangzhou, Guangdong 510240)

    Abstract Objective: To investigate the correlation between cyclooxygenase2 (COX2) and expression of vascular endothelial growth factor ( VEGF ) in hepatocellular carcinoma cell line BEL7402. Methods: Cultured hepatocellular carcinoma cell lines BEL7402, extracted mRNA from these cells, measured the VEGF levels in the culture medium of these cells using ELISA, and identified the expression of COX2 and VEGF by RTPCR. Results: The cultured hepatocellular carcinoma cell lines BEL7402 can express both COX2 and VEGF at same time. Celecoxib, COX2 inhibitor, could decrease the expression of VEGF. And the levels of VEGF were correlated with COX2 expression. COX2 inhibited the expression of VEGF at mRNA level. Conclusions: COX2 plays an important role in the VEGF secretion and synthesis. Therefore, it has an effect on the angiogenesis and tumor growth. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/15/83.htm

您现在查看是摘要页，全文长 7668 字符。