HPLC法测定注射用加替沙星中加替沙星的含量
加替沙星,,加替沙星;注射用加替沙星;反相高效液相色谱法,1仪器与试药,2方法与结果,3讨论,参考文献
摘 要 目的 测定注射用加替沙星中加替沙星的含量。方法 采用反相高效液相色谱法,色谱柱为C18,ψ(乙腈∶甲醇∶醋酸-三乙胺缓冲液∶十二烷基磺酸钠)=30∶40∶30∶0.25 g为流动相。流速为1.0 mL/min,检测波长为290 nm。结果 加替沙星在8.05~161.0 μg/mL范围内线性关系良好(r=0.9996),平均回收率为99.9%,RSD为0.4%。结论 本法准确、灵敏、可靠,能有效地控制该制剂的质量。关键词 加替沙星;注射用加替沙星;反相高效液相色谱法
HPLC determination of gatifloxacin in gatifloxacin for injection
WANG Changding,FU Xiujuan ,LIN Lizhen
(Hainan Sanye Pharmaceutical Co., Ltd., Haikou 570226, China)
Abstract Objective Assay gatifloxacin in gatifloxacin for injection. Methods RPHPLC method was applied in assay with C18 column, with the mobile phase of ψ(acetonitrile: methanol: acetic acidtriethylamine buffer: sodium dodecyl sulfate)=30∶40∶30∶0.25 and detection wavelength of 290 nm. Result Gatifloxacin showed linear correlation in the range of 8.05~161.0 mg/mL (r=0.9996) with recovery of 99.9% and RSD of 0.4%. Conclusion The method is accurate and reliable, and can be used in quality control of gatifloxacin for injection.
Key words gatifloxacin; gatifloxacin for injection; RPHPLC
注射用加替沙星(gatifloxacin)为氟喹诺酮类抗菌药 ......
您现在查看是摘要页,全文长 4532 字符。