糖尿病_____]_糖尿病,_2型;_凋亡;

六味地黄丸对OLETF大鼠胰腺凋亡相关基因bcl2和Bax表达的影响

http://www.100md.com 《中西医结合学报》 2005年第6期

糖尿病,,],糖尿病,2型;,凋亡;,基因,bcl2;,基因,Bax;,六味地黄汤;,大鼠,OLETF,1材料和

     [摘要] 目的：探讨六味地黄丸对自发性2型糖尿病大鼠胰腺组织凋亡相关基因bcl2和Bax表达的影响。方法：OLETF大鼠(自发性2型糖尿病)40只，随机分为六味地黄丸治疗组和模型组，每组20只；另同系LETO大鼠(非糖尿病)10只作为正常对照组。六味地黄丸治疗组于大鼠8周龄起，用六味地黄丸按2.4 g·kg1·d1灌胃，余组用等量蒸馏水灌胃。每周记录大鼠体质量；采用口服葡萄糖耐量试验监测血糖；定期处死大鼠，分离胰腺并称重；采用逆转录聚合酶链反应检测bcl2和Bax在胰腺组织中的表达。结果：大鼠40周龄时，六味地黄丸治疗组bcl2 mRNA的表达水平为(1.25±0.07)，较模型组(1.01±0.16)明显增高(P<0.01)；Bax mRNA的表达水平为(0.57±0.11)，较模型组(1.18±0.28)有明显降低(P<0.01)。六味地黄丸治疗组的胰腺/体重比，较模型组增高，但差异无统计学意义。六味地黄丸治疗组的糖负荷能力明显高于模型组(P<0.05或P<0.01)。结论：六味地黄丸在转录水平可上调bcl2 mRNA的表达，下调Bax mRNA的表达，可能具有抗细胞凋亡的作用。

    [关键词] 糖尿病, 2型; 凋亡; 基因, bcl2; 基因, Bax; 六味地黄汤; 大鼠, OLETF

    Effects of Liuwei Dihuang Pills on expressions of apoptosisrelated genes bcl2 and Bax in pancreas of OLETF rats

    XUE YaoMing, LUO Ren, ZHU Bo, ZHANG Yan, PAN YongHua, LI ChenZhong

    (Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China; Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China)

    ABSTRACT Objective: To investigate the effects of Liuwei Dihuang Pills (LWDHP) on expressions of apoptosisrelated genes bcl2 and Bax in pancreas of OLETF rats. Methods: Forty male OLETF rats were randomly divided into LWDHPtreated group and untreated group. Another ten male LETO rats were included in normal control group. OLETF rats in the LWDHPtreated group were given LWDHP (2.4 g·kg1·d1) orally since the age of 8 weeks and the rats in the other two groups were given distilled water orally. Body weights of rats were recorded weekly and blood glucose concentration was determined by oral glucose tolerance test (OGTT). Pancreas weights were recorded after rats were killed and the expression levels of bcl2 mRNA and Bax mRNA were detected by reverse transcriptasepolymerase chain reaction (RTPCR). Results: In the LWDHPtreated group, the expression of bcl2 mRNA in the pancreas of rats at the age of 40 weeks (1.25±0.07) was much higher than that in the untreated group (1.01±0.16), P<0.01. Bax mRNA level in the LWDHPtreated group (0.57±0.11) was obviously lower than that in the untreated group (1.18±0.28), P<0.01. There was no significant difference of pancreastobody weight ratios between the LWDHPtreated group and the untreated group. The ability of glucose tolerance was improved in the LWDHPtreated group. Conclusion: LWDHP can upregulate the expression of bcl2 and downregulate the expression of Bax at transcription level, which maybe contribute to the antiapoptosis effects of LWDHP. ......

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