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食管癌基因表达谱及其与细胞分化的相关性研究
http://www.100md.com 《第四军医大学学报》 2006年第9期
食管肿瘤,,食管肿瘤;基因芯片;基因表达谱;细胞分化,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Study on gene expression profiles and their relation to cell differentiation in human esophageal squamous cell carcinoma

    LI Pei, LING ZhiQiang, YANG HongYan, HUANG YouTian, ZHAO JiMin, DONG ZiMing

    Department of Pathophysiology, Basic Medical College, Zhengzhou University, Zhengzhou 450052, China, Institute of Bioengineering, Zhejiang Academy of Medical Sciences, Hangzhou 310013,China

    【Abstract】 AIM: To investigate the gene expression profiles and to screen the genes related to cell differentiation in primary esophageal cancer, which is helpful to search and locate cell differentiationassociated genes and deepen the understanding of the molecular mechanism of its tumourgenesis. METHODS: cDNA microarray was used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 cases of human esophageal squamous cell carcinoma (ESCC). The results were analyzed by bioinformatics. RESULTS: Among the 886 target genes, 110 (12.42%) genes were differentially expressed commonly at least twice in all 15 samples. There was a correlation between 16 (1.81%) genes and cell differentiation, of which 9 (1.02%) were upregulated and 7 (0.79%) downregulated. CONCLUSION: Many ESCCassociated genes were screened by highthroughput gene chip method; validating their cellular functions will help to identify the key genes or pathway responsible for ESCC, which might be used as diagnostic markers and therapeutic targets for ESCC. ......

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