一氧化氮合酶__(期刊论文)

T2毒素对软骨细胞合成一氧化氮的影响

http://www.100md.com 《西安交通大学学报》 2006年第2期

一氧化氮合酶,,T2毒素；软骨细胞；一氧化氮；一氧化氮合酶,1材料与方法,2结果,3讨论,参考文献：

     作者简介：杨占田(1961)，男(汉族)，主管技师.

    摘要：目的探讨T2毒素对软骨细胞合成一氧化氮(NO)的影响。方法胎儿软骨细胞体外培养，用MTT法检测软骨细胞存活率；用Griess重氮化法测定软骨细胞培养上清液中NO含量；用Western blot检测软骨细胞一氧化氮合酶(iNOS)的表达。结果 T2毒素在质量浓度在1-2000μg/L的范围内，软骨细胞存活率对其呈较典型的浓度依赖关系和时间依赖关系，而且随着作用时间的延长, 浓度依赖关系更为明显； T2毒素刺激软骨细胞分泌NO和表达iNOS蛋白。结论 T2毒素引起的软骨细胞损伤和生长抑制与T2毒素刺激软骨细胞表达iNOS蛋白和分泌NO增多有关。

    关键词：T2毒素；软骨细胞；一氧化氮；一氧化氮合酶

    Effects of T2 toxin on nitric oxide production in chondrocytesYang Zhantian, Chen Jinghong, Chu Yonglie, Cao Junling, Wang Zhilun, Shi Zhongli, Guo Xiong

    (1. Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education;

    2. Department of Microbiology, Medical School of Xian Jiaotong University, Xian 710061, China)

    ABSTRACT: Objective To investigate the effects of T2 toxin on nitric oxide (NO) production and nitric oxide synthase (iNOS) expression in chondrocytes. Methods Human chondrocytes cultured in vitro were treated with different concentrations of T2 toxin in different time (1-5 day). Cell viability of the treated cells was measured by MTT assay; The levels of NO in culture media were detected by colorimetric method of Griess assay; iNOS protein was measured by Western blot. Results Dosedependent and time dependent effects of T2 toxin were found on T2 toxin treated chondrocytes within a certain range of concentration (1-2000μg/L) and a periods of time (1-5 day); The levels of NO in T2 toxin treated culture media were higher than that of normal control group; Overexpression of iNOS was detected in T2 toxin treated cells. Conclusion T2 toxin can enhance NO production and upregulate expression of iNOS. NO plays an important role in T2 toxin induced growth inhibitory and toxic effects. ......

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