survivin启动子的克隆及在HeLa细胞中控制GFP特异性表达
遗传学,,survivin；启动区(遗传学)；基因疗法；GFP,0引言,1材料和方法,2结果,3讨论,【参考文献】
Molecular cloning of survivin gene promoter and detecting specific expression of GFP in HeLa cellWU Bing ，LIN Fang，LIU Li，WANG Yan，ZHANG HuiZhong
Department of Clinical Diagnosis，Tangdu Hospital，Fourth Military Medical University ，Xian 710038，China
【Abstract】AIM: To molecular clone survivin gene promoter and detect the activity of survivin promoter in Hela cells. METHODS: (1) Approximately 1 kb of the survivin gene promoter region was generated by polymerase chain reaction using HeLa cell genomic DNA as a template and cloned into the vector pGEMTeasy to generate the plasmid survivinTeasy (T/Surp) .The plasmid was transformed into JM109 E.coli competent cells and positive clone was selected .The survivin gene promoter was further confirmed by DNA sequence analysis. (2)The survivin gene promoter in T vector was cut with SacⅠ and HindⅢ enzyme and received ，then religated with pEGFP-N1 vector which not include CMV promoter to generate the plasmid pEGFP-N1/ Surp. (3) The purified pEGFP-N1/ Surp was transfected into HeLa cell and ECV304 ......
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