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水飞蓟素脂质体对小鼠CCl4急性肝损伤保护作用的实验研究
http://www.100md.com 《新疆医科大学学报》2006年第5期
水飞蓟素脂质体,,水飞蓟素脂质体;,益肝灵片;,肝损伤,摘要,关键词,1材料与方法,2结果,3讨论,参考文献
     摘要: 目的:研究水飞蓟素脂质体(LSIL)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:昆明种小鼠80只,随机分为空白组、CCl4急性肝损伤模型组及LSIL低、中、高剂量组和益肝灵(TSIL)低、中、高剂量组共8组,测量并比较各组小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平及肝组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPX)水平和丙二醛(MDA)含量,并观察各组小鼠肝组织病理学改变。结果:与CCl4急性肝损伤模型组相比,LSIL各剂量组血清ALT、AST水平明显降低(P<0.05~0.01),LSIL各剂量肝组织匀浆GSHPX水平及LSIL高剂量组SOD水平明显升高(P<0.05~0.01),并具有剂量依赖性,LSIL高剂量组肝组织匀浆MDA含量明显降低(P<0.01)。LSIL对ALT、SOD、GSHPX水平的作用优于相应剂量TSIL组(P<0.05~0.01)。组织学观察LSIL中、大剂量组的肝损伤明显轻于模型组和相应剂量TSIL组。结论:LSIL对小鼠CCl4肝损伤有明显的保护作用,且其保护作用明显优于TSIL。

    关键词: 水飞蓟素脂质体; 益肝灵片; 肝损伤

    The protective effect of silymarin liposomes against

    experimental liver injury in mice

    XU Hongxia, GAO Xiaoli

    (Department of Pharmacy, College of Pharmacy, Xinjiang Medical University, Urumqi 830054, China)

    Abstract: Objective: To investigate the protective effect of silymarin liposomes(LSIL) on the CCl4induced acute liver injury in mice. Methods: Kun ming mice were randomly divided into normal control group, model control group, high, middle and low dose groups of LSIL and high, middle and low dose groups of TSIL. The model of hepatic damage were induced by CCl4. The Alanine aminotransferase(ALT), Aspartate aminotransferase(AST) levels in serum , malondialdehyde(MDA) content, superoxide dismutase(SOD), glutathione peroxidase(GSHPX) levels in liver homogenate were examined and dimensions of hepatic damage in the histopathology were observed. Results: LSIL could inhibit the rising of serum ALT, AST levels caused by CCl4 in doseresponse manner(P<0.05~0.01). Meanwhile, LSIL also decreased MDA content(P<0.01) and prevented the reduction of SOD, GSHPX levels in the liver homogenate(P<0.05~0.01). The effects of LSIL on the ALT level in serum and SOD, GSHPX levels in liver homogenate against experimental liver injury in mice were more powerful than that of TSIL of the same dose(P<0.05~0.01). The liver pathological observation showed that the liver injury of LSIL (150,300 mg/kg.d) were significantly less than that of the model group and TSIL of the same dose. Conclusion: The protective effect of LSIL on the CCl4induced acute liver injury in mice was more potent than that of TSIL in the same dose. ......

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