HSP70-肿瘤肽复合物的纯化及其对肝癌细胞株HepG-2增殖的作用
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隋春阳, 李航宇, 胡 勇, 宗志红, 李洪秀,
HSP70-肿瘤肽复合物;纯化;肝癌;HepG-2细胞系;Westernblot;MTT隋春阳,李航宇,胡勇,宗志红,李洪秀,郭仁宣.HSP70-肿瘤肽复合物的纯化及其对肝癌细胞株HepG-2增殖的作
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隋春阳, 胡勇, 李洪秀, 郭仁宣, 中国医科大学第一临床学院普外二科 辽宁省沈阳市 110001
李航宇, 中国医科大学第二临床学院微创外科 辽宁省沈阳市 110001
宗志红, 中国医科大学生化教研室 辽宁省沈阳市 110001隋春阳, 2004年中国医科大学博士, 主治医师, 主要从事肝胆外科疾病的研究.
通讯作者: 郭仁宣, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院普外科. sui-chun@163.com
电话: 024-23256666
收稿日期: 2006-05-08 接受日期: 2006-05-22
Purification of heat shock protein 70 peptide complex and its effect on proliferation of HepG-2 cells
Chun-Yang Sui, Hang-Yu Li, Yong Hu, Zhi-Hong Zong, Hong-Xiu Li, Ren-Xuan Guo
Chun-Yang Sui, Yong Hu, Hong-Xiu Li, Ren-Xuan Guo, the Second Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China
Hang-Yu Li, Department of Minimally Invasive Surgery, the Second Affiliated Hospital of China Medical University, Shenyang 110001, China
Zhi-Hong Zong, Department of Biochemistry, China Medical University, Shenyang 110001, China
Correspondence to: Dr. Ren-Xuan Guo, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China. sui-chun@163.com
Received: 2006-05-08Accepted: 2006-05-22
Abstract
AIM: To separate and purify heat shock protein 70 (HSP70) peptide complex from hepatocellular carcinoma (HCC) tissues by fast protein liquid chromatography (FPLC), and investigate its effect on the proliferation of HepG-2 cells.
METHODS: The mixture of proteins was derived from HCC tissues by means of splitting and centrifugation. Then the proteins were purified by affinity chromatography on concanavalin A-Sepharose and ion exchange chromatography with DEAE-Sephacel. The obtained protein was identified by SDS-PAGE and Western blot for its molecular weight and property. Bradford method was used to measure the concentration of the protein. The growth of HepG-2 cells, which were stimulated by HSP-70 peptide complex (0 ......
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