磷脂酰肌醇3激酶/蛋白激酶B信号转导通路在重症急性胰腺炎中性粒细胞活化中的作用
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张 成, 栾正刚, 葛春林, 郭仁宣
磷脂酰肌醇3激酶;重症急性胰腺炎;中性粒细胞;信号转导张成,栾正刚,葛春林,郭仁宣.磷脂酰肌醇3激酶,蛋白激酶B信号转导通路在重症急性胰腺炎中性粒细胞活化中的作用. 世界华人消化杂志200
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张成, 葛春林, 郭仁宣, 中国医科大学附属第一医院普外二科 辽宁省沈阳市 110001
栾正刚, 中国医科大学附属第一医院ICU室 辽宁省沈阳市 110001张成, 2004年中国医科大学普通外科博士生, 主要从事重症急性胰腺炎发病机制方面的研究.
通讯作者: 葛春林, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院普外二科. cz1791@163.com
电话: 024-83283330
收稿日期: 2006-05-10 接受日期: 2006-06-08
Role of phosphatidylinositol 3-kinase/protein kinase B signal transduction pathway in activation of neutrophils during severe acute pancreatitis
Cheng Zhang, Zheng-Gang Luan, Chun-Lin Ge, Ren-Xuan Guo
Cheng Zhang, Chun-Lin Ge, Ren-Xuan Guo, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Zheng-Gang Luan, Department of Intensive Care Unit, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Dr. Chun-Lin Ge, Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, Chin. cz1791@163.com
Received: 2006-05-10Accepted: 2006-06-08
Abstract
AIM:To investigate the role of phosphatidy-linositol 3-kinase/protein kinase B (PI3K/PKB) signal transduction pathway in the activation of neutrophils during severe acute pancreatitis (SAP).
METHODS: Thirty male health adult Sprague Dawley rats were averagely randomized into group A, B and C. SAP model was established in group B and C by retrograde injection of 50 g/L sodium taurocholate into the pancreatic and biliary duct. The rats in group C were treated with wortmannin 4 h before modeling, and those in group A served as the controls. All the rats were killed 6 h after modeling, and the neutrophils were isolated by density gradient centrifugation. The serum levels of tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b) were analyzed by enzyme-linked immunosorbent assay (ELISA). The expression of TNF-a and IL-1b mRNA were detected by RT-PCR and the activity of phosphorylated PKB (p-PKB) was examined by Western blot ......
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