单一质粒四环素系统调控IL-24表达对胃癌细胞系的作用
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林宁晶, 朱 军
四环素调控;白细胞介素24;胃癌;增殖;凋亡,林宁晶,朱军,通讯作者:,电话:,传真:,收稿日期:,接受日期:,Interleukin
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林宁晶, 朱军, 北京大学临床肿瘤学院肿瘤内科 北京市 100036林宁晶, 2001年北京大学第一医院硕士毕业, 2006年北京大学临床肿瘤学院博士毕业, 主要从事晚期实体瘤的治疗.
国家“十五”科技攻关计划项目资助, No. 2002BA711A06
通讯作者: 朱军, 100036, 北京市海淀区阜成路52号, 北京大学临床肿瘤学院肿瘤内科. zhujun@csco.org.cn
电话: 010-88196118 传真: 010-88122437
收稿日期: 2006-05-20 接受日期: 2006-06-09
Interleukin-24 selectively induces growth suppression and apoptosis in human gastric cancer cells transfected with a single tetracycline-regulatable plasmid
Ning-Jing Lin, Jun Zhu
Ning-Jing Lin, Jun Zhu, Department of Internal Oncology, Peking University School of Oncology, Beijing 100036, China
Supported by the National Program for Key Science and Technology Projects, No. 2002BA711A06
Correspondence to: Dr. Jun Zhu, Department of Internal Oncology, Peking University School of Oncology, 52 Fucheng Road, Haidian District, Beijing 100036,China. zhujun@csco.org.cn
Received: 2006-05-20 Accepted: 2006-06-09
AbstractAIM:To estimate the tumor-suppressive activity of interleukin-24 (IL-24) in gastric cancer cells in vitro and the regulation of tetracycline in a single plasmid pCEP4-tetR-IL-24.
METHODS: A single plasmid pCEP4-tetR-IL-24 was constructed which contained the TetR and TetO. The cells were transfected with pCEP4-tetR-IL-24 using lipofectamine 2000. The expression of IL-24 was detected by Western blotting. The suppression of cellular growth was determined by MTT assay and trypan blue rejection. The induction of apoptosis was analyzed by Annexin Ⅴ staining.
RESULTS: IL-24 protein was induced to express in gastric cancer cells (MGC803 and BGC823) and normal cells (GES-1) after transfection with pCEP4-tetR-IL-24. In the MGC803 and BGC823 cells, cellular growth was inhibited more efficiently (P < 0.01, 0.001-0.006), and the percentage of apoptosis cells was higher after doxycline induction (23.5%/25.6%→33.8%/36.7%, P < 0 ......
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