野生型K-ras2诱导结肠癌细胞基因表达谱改变的特征分析
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李 红, 曹厚法, 万 军, 李 园, 朱美玲,
野生型K-ras2;结肠癌;基因芯片,李红,曹厚法,万军,李园,朱美玲,韩为东,通讯作者:,电话:, 传真:,收
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李红, 曹厚法, 中国人民解放军第301医院特需住院部二科 北京市 100853
万军, 李园, 朱美玲, 中国人民解放军第301医南楼消化科 北京市 100853
韩为东, 中国人民解放军第301医院分子生物学室 北京市 100853李红, 1991年哈尔滨医科大学学士, 1998年军医进修学院老年消化专业博士, 副主任医师, 主要从事消化道肿瘤的分子生物学与胃肠肿瘤的发病机制的研究.国家自然科学基金青年基金项目, No. 302000326
通讯作者: 李红, 100853, 北京市复兴路28号, 中国人民解放军第301医院特需住院部二科. lihongam@hotmail.com
电话: 010-68295590 传真: 010-68295592
收稿日期: 2006-04-13 接受日期: 2006-05-22
Screening of genes differentially expressed in Caco2 cells transfected with wild type K-ras2 by cDNA microarray
Hong Li, Hou-Fa Cao, Jun Wan, Yuan Li, Mei-Ling Zhu, Wei-Dong Han
Hong Li, Hou-Fa Cao, Inpatient Department of Special Need Treatment, General Hospital of Chinese PLA, Beijing 100083, China
Jun Wan, Yuan Li, Mei-Ling Zhu, Department of South Building Gastroenterology, General Hospital of Chinese PLA, Beijing 100853, China
Wei-Dong Han, Department of Molecular Biology, General Hospital of Chinese PLA, Beijing 100083, China
Supported by National Natural Science Foundation for the Youth of China, No. 30200326
Correspondence to: Hong Li, Inpatient Department of Special Need Treatment, General Hospital of Chinese PLA, Beijing 100853, China. lihongam@hotmail.com
Received: 2006-04-13Accepted: 2006-05-22
AbstractAIM:To investigate the genes differentially expressed in human colon carcinoma cell line Caco2 transfected with wild type K-ras2-expressing plasmid and further elucidate the potential molecular biological function of wild type K-ras2.
METHODS:Sequence specific primers were designed and synthesized, and the wild type K-ras2 DNA fragment was amplified with polymerase chain reaction (PCR) technique. The expressive vector of pCI-neo-K-ras2 was constructed by routine molecular biological methods. cDNA microarray technique was employed to detect the mRNA expression in Caco2 cells transfected with pCI-neo-K-ras2 and pCI-neo, respectively, using lipofectamine ......
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