上皮型钙粘附蛋白和层粘连蛋白在妊娠滋养细胞疾病中的表达及意义
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刘景瑜 ,安瑞芳 ,张 晨 ,王 姝
妊娠滋养细胞疾病;葡萄胎;侵蚀性葡萄胎;绒毛膜癌;层粘连蛋白
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摘要：目的 探讨上皮型钙粘附蛋白(Ecad)和层粘连蛋白(LN)在妊娠滋养细胞疾病(GTD)中的表达及意义。方法 应用免疫组织化学(SP法)检测74例GTD中Ecad和LN的表达情况。结果 在GTD中，Ecad的正常表达率和LN强表达率分别为37.8%(28/74)和64.86%(48/74)，显著低于正常绒毛组织。在葡萄胎(HM)、侵蚀性葡萄胎(IM)及绒毛膜癌(CC)中Ecad的正常表达率分别为53.3%、31.25%和16.67%；LN的强表达率分别为80%、56.25%和50%。随着GTD恶性程度的增加，Ecad的正常表达率及LN的强表达率逐渐降低，且与正常绒毛组织有显著差异。结论 Ecad及LN的表达与GTD的发生、恶变及侵袭有关系；对Ecad与LN表达的监测有助于预测GTD的发展趋势及提示治疗效果。
关键词：妊娠滋养细胞疾病；葡萄胎；侵蚀性葡萄胎；绒毛膜癌；层粘连蛋白
The expression of Ecadherin and Laminin in gestational trophoblastic diseases and their significance
Liu Jingyu, An Ruifang, Zhang Chen, Wang Shu
(Department of Obstetrics and Gynecology, Nan Jing Drum Tower Hospital, Medical School of
Nan Jing University, Nanjing 210008；Department of Obstetrics and Gynecology,First Affiliated Hospital, Medical School of Xian Jiaotong University, Xian 710061, China)
ABSTRACT：Objective To explore the expressions of Ecadherin(Ecad) and Laminin(LN) in gestational trophoblastic diseases(GTD) and their significance. Methods The expression of Ecadherin and LN in 74 cases gestational diseases was detected by immunohistochemical (SP) method. Results The normal expression rates of Ecad and the strong expression rates of LN in GTD were 37.8% (28/74) and 64.86% (48/74), respectively, and were significantly lower than those in normal villus tissue. In HM (hydatidiform mole), IM (invasive mode) and CC(choriocarcinoma), the normal expression rates of Ecad were 53.3%, 31.25% and 16.67%, respectively; the strong expression rates of LN were 80%, 56.25% and 50%, respectively. With the development of GTD malignancy, the normal expression rates of Ecad and the strong expression rates of LN declined gradually and were significantly different from those of normal villus. The decreasing expression of the both were associated with the development, malignance and invasiveness of GTD. In GTD, there were no significant relationship between the normal expression of Ecad and the strong expression of LN. Conclusion The expression of the Ecad and LN are associated with the development, malignance and invasiveness of GTD. The combination of them is helpful for the diagnosis of GTD, and its valuable to predict the progress trend and prognosis of GTD.
KEY WORDS: gestational trophoblastic disease; hydatidiform mole; invasive toole; choriocarcinoma; Ecadherin; laminin
妊娠滋养细胞疾病(gestational trophoblastic disease, GTD)是一组来源于胎盘绒毛滋养细胞的疾病，其中包括葡萄胎(hydatidiform mole, HM)，侵蚀性葡萄胎(invasive mole, IM)和绒毛膜癌(choriocarcinoma, CC)。滋养细胞生物行为具有某些肿瘤细胞的特性，其异常增生导致GTD的机理仍不清楚，但可以肯定的是GTD的发生是多因素作用的复杂过程。我们采用免疫组织化学的方法，观察HM、IM及CC组织中上皮型钙粘附蛋白(Ecad)及层粘连蛋白(LN)的表达情况，结合临床资料分析，旨在探讨Ecad及LN与GTD的发生、发展及侵袭转移的关系，进而揭示其临床意义及应用价值。
1 材料与方法
1.1 临床资料 收集西安交通大学医学院第一附属医院妇科1990年1月至2000年12月妊娠滋养细胞疾病组织标本74例，其中HM 30例，均为首次清宫标本，IM 32例，CC l2例，上述标本均用10%(体积分数)中性福尔马林固定，石蜡包埋，切片厚5μm，分别作HE和SP免疫组化染色。主要试剂有鼠抗人Ecad单克隆抗体(Onco gene公司)，鼠抗人LN单克隆抗体(Santa Cruz生化公司)，SP试剂盒(ZYMED公司)。同时取正常早期妊娠绒毛组织标本20例作为对照组。
1.2 结果判定 Ecad为细胞膜表达蛋白，正常组织中Ecad均匀一致的呈线性表达于细胞膜上 ......
摘要：目的 探讨上皮型钙粘附蛋白(Ecad)和层粘连蛋白(LN)在妊娠滋养细胞疾病(GTD)中的表达及意义。方法 应用免疫组织化学(SP法)检测74例GTD中Ecad和LN的表达情况。结果 在GTD中，Ecad的正常表达率和LN强表达率分别为37.8%(28/74)和64.86%(48/74)，显著低于正常绒毛组织。在葡萄胎(HM)、侵蚀性葡萄胎(IM)及绒毛膜癌(CC)中Ecad的正常表达率分别为53.3%、31.25%和16.67%；LN的强表达率分别为80%、56.25%和50%。随着GTD恶性程度的增加，Ecad的正常表达率及LN的强表达率逐渐降低，且与正常绒毛组织有显著差异。结论 Ecad及LN的表达与GTD的发生、恶变及侵袭有关系；对Ecad与LN表达的监测有助于预测GTD的发展趋势及提示治疗效果。
关键词：妊娠滋养细胞疾病；葡萄胎；侵蚀性葡萄胎；绒毛膜癌；层粘连蛋白
The expression of Ecadherin and Laminin in gestational trophoblastic diseases and their significance
Liu Jingyu, An Ruifang, Zhang Chen, Wang Shu
(Department of Obstetrics and Gynecology, Nan Jing Drum Tower Hospital, Medical School of
Nan Jing University, Nanjing 210008；Department of Obstetrics and Gynecology,First Affiliated Hospital, Medical School of Xian Jiaotong University, Xian 710061, China)
ABSTRACT：Objective To explore the expressions of Ecadherin(Ecad) and Laminin(LN) in gestational trophoblastic diseases(GTD) and their significance. Methods The expression of Ecadherin and LN in 74 cases gestational diseases was detected by immunohistochemical (SP) method. Results The normal expression rates of Ecad and the strong expression rates of LN in GTD were 37.8% (28/74) and 64.86% (48/74), respectively, and were significantly lower than those in normal villus tissue. In HM (hydatidiform mole), IM (invasive mode) and CC(choriocarcinoma), the normal expression rates of Ecad were 53.3%, 31.25% and 16.67%, respectively; the strong expression rates of LN were 80%, 56.25% and 50%, respectively. With the development of GTD malignancy, the normal expression rates of Ecad and the strong expression rates of LN declined gradually and were significantly different from those of normal villus. The decreasing expression of the both were associated with the development, malignance and invasiveness of GTD. In GTD, there were no significant relationship between the normal expression of Ecad and the strong expression of LN. Conclusion The expression of the Ecad and LN are associated with the development, malignance and invasiveness of GTD. The combination of them is helpful for the diagnosis of GTD, and its valuable to predict the progress trend and prognosis of GTD.
KEY WORDS: gestational trophoblastic disease; hydatidiform mole; invasive toole; choriocarcinoma; Ecadherin; laminin
妊娠滋养细胞疾病(gestational trophoblastic disease, GTD)是一组来源于胎盘绒毛滋养细胞的疾病，其中包括葡萄胎(hydatidiform mole, HM)，侵蚀性葡萄胎(invasive mole, IM)和绒毛膜癌(choriocarcinoma, CC)。滋养细胞生物行为具有某些肿瘤细胞的特性，其异常增生导致GTD的机理仍不清楚，但可以肯定的是GTD的发生是多因素作用的复杂过程。我们采用免疫组织化学的方法，观察HM、IM及CC组织中上皮型钙粘附蛋白(Ecad)及层粘连蛋白(LN)的表达情况，结合临床资料分析，旨在探讨Ecad及LN与GTD的发生、发展及侵袭转移的关系，进而揭示其临床意义及应用价值。
1 材料与方法
1.1 临床资料 收集西安交通大学医学院第一附属医院妇科1990年1月至2000年12月妊娠滋养细胞疾病组织标本74例，其中HM 30例，均为首次清宫标本，IM 32例，CC l2例，上述标本均用10%(体积分数)中性福尔马林固定，石蜡包埋，切片厚5μm，分别作HE和SP免疫组化染色。主要试剂有鼠抗人Ecad单克隆抗体(Onco gene公司)，鼠抗人LN单克隆抗体(Santa Cruz生化公司)，SP试剂盒(ZYMED公司)。同时取正常早期妊娠绒毛组织标本20例作为对照组。
1.2 结果判定 Ecad为细胞膜表达蛋白，正常组织中Ecad均匀一致的呈线性表达于细胞膜上 ......
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