疏血通对脑缺血再灌注损伤的保护作用及其对抗氧化指标的影响
疏血通；再灌注损伤；抗氧化系统,,疏血通；再灌注损伤；抗氧化系统,1材料与方法,2结果,3讨论,【参考文献】
【摘要】 目的 探讨疏血通对沙土鼠缺血再灌注脑组织的保护作用及其机制。方法 48只成年雄性沙土鼠随机分成假手术组、缺血再灌注组和治疗组，阻断双侧颈总动脉7min后使血液复流，制成沙土鼠脑缺血再灌注模型。每组随机取8只沙鼠于再灌注后24h处死，分离出海马区及额顶部脑组织，作脑组织匀浆。测定脑组织匀浆中的SOD、MDA的含量，同时对再灌注后12h、3天、7天海马区组织的病理学改变进行观察。然后比较假手术组、缺血再灌注组和治疗组上述指标的变化与不同。结果 缺血再灌注后12h治疗组较缺血再灌注组海马CA1区神经元变性、坏死程度明显减轻，随再灌注时间的推移两组变性、坏死及形态不规则的神经元均逐渐增多；超微结构显示治疗组细胞器、细胞核及细胞内膜系统的损伤性改变较缺血再灌注组明显减轻。治疗组SOD的含量明显高于缺血再灌注组(P<0.05)，而缺血再灌注组SOD含量明显低于假手术组(P<0.05)；治疗组MDA的含量则明显低于缺血再灌注组(P<0.05)，而缺血再灌注组MDA的含量明显高于假手术组(P<0.05)。结论 疏血通可以提高缺血再灌注脑组织内SOD的含量、对抗自由基、抑制脂质过氧化反应，从而对缺血再灌注脑组织的损伤发挥保护作用。【关键词】 疏血通；再灌注损伤；抗氧化系统
The protective effect of Shuxuetong on cerebral ischemiareperfusion injury and influence to antioxidative indexes
ZHANG Dongjun，LI Zhiyuan，DING Li-gong，et al.Department of Neurology，Qilu Hospital of Shandong University，Jinan 250012，China
【Abstract】 Objective To study the protective effect and mechanism of Shuxuetong on gerbil brain tissue in the area of ischemiareperfusion.Methods 48 male gerbils were divided into three groups:sham operation group，ischemiareperfusion group and treatment group.Cerebral ischemiareperfusion in gerbils was made by transient clipping bilateral common carotid arteries for 7 minute.8 gerbils were selected at random and put to death at 24 hours after reperfusion in each group.Hippocampal and frontalparietal tissue were separated for making homogenate.Contents of SOD and MDA of brain tissue were measured at 24 hours after reperfusion.Pathological changes in the hippocampal tissue were observed at different reperfusion time (12th hours ......
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