白藜芦醇；全细胞膜片钳；钙电流_

白藜芦醇对豚鼠心室肌细胞L型钙通道的影响

http://www.100md.com 《第四军医大学学报》 2006年第13期

白藜芦醇；全细胞膜片钳；钙电流,,白藜芦醇；全细胞膜片钳；钙电流,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effects of resveratrol on Ltype calcium current in isolated ventricular myocytes of guinea pig

    YI FangFang, TANG QiZhu, FENG SiTing, WANG Teng

    Department of Cardiology, Peoples Hospital, Wuhan University, Wuhan 430060, China

    【Abstract】 AIM: To investigate the effects of resveratrol on the Ltype calcium current (ICaL) in isolated ventricular myocytes of guinea pig. METHODS: Single ventricular cell was obtained from healthy guinea pig heart using enzymatic dissociation methods. The wholecell patch clamp was used to record the ICaL following the administration of resveratrol at 1, 50, 100 μmol/L. RESULTS: ① Resveratrol reduced the ICaL in a concentrationdependent manner, but it had no effect on IV shape of ICaL. ② Resveratrol markedly shifted the steadystate activation curve of ICaL to the right, and V1/2 value increased from (-18.97±4.08) mV in control to (-15.97±4.13) mV in the presence of resveratrol (n=7, P=0.02), the κ value from (3.64±0.56) to (3.82±0.60) (n=7，P=0.67). ③ Inactivation curve of ICaL was not affected significantly. ④ The time constant of reactivation of ICaL was reduced from (98.50±9.00) ms to (54.53±4.27) ms (n=6 ......

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