抗血管内皮生长因子抗体对肝癌的放射增敏作用
肝肿瘤;放射疗法;抗VEGF单抗;放射增敏,,肝肿瘤;放射疗法;抗VEGF单抗;放射增敏,0引言,1材料和方法,2结果,3讨论,【参考文献】
Radiosensibility of human hepatocellular carcinoma xenografts enhanced by antivascular endothelial growth factor monoclonal antibodyZHENG QingPing, CHEN LongHua, SHI YuSheng
Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
【Abstract】 AIM: To investigate the inhibiting effects of combining antivascular endothelial growth factor (VEGF) monoclonal antibody (mAb) therapy with radiotherapy on the growth of hepatocellular carcinoma xenografts in vivo. METHODS: Human hepatocellular carcinoma cell line, SMMC7721, was implanted sc in mice. The mice with xenografts were divided into 4 groups, radiation (RT) 20 Gy group, RT 20 Gy plus mAb group, RT 30 Gy plus mAb group and control group. AntiVEGF mAb was injected ip on alternate days for a total of 6 injections at a dosage of 50 μg/mouse. Single radiation doses were given 24 h after the 4th injection of mAb. Tumor diameters were measured and tumor volume was calculated. Mice were sacrificed 2 wk after the 6th injection and the intratumoral microvessel density was determined by counting the stained vessels with immunohistochemistry. RESULTS: Radiation significantly reduced the growth and the microvessel density of tumors. The inhibitory effects were more significant when radiation was combined with antiVEGF mAb, and RT 30 Gy plus mAb was more effective than RT 20 Gy plus mAb. For RT 20 Gy group, RT 20 Gy plus mAb group and RT 30 Gy plus mAb group, the inhibitory rates of tumor weight were 75.3%, 83.9% and 94.7%, respectively. CONCLUSION: AntiVEGF mAb enhances the radiosensibility of human hepatocellular carcinoma xenografts.The strategy is one of the approaches for hepatocellular carcinoma treatment. ......
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