Cyclin E表达失调导致人类结肠癌染色体不稳定
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陈剑英, 黄文广, 陶凯雄, 王国斌
CyclinE;人类结肠癌;CIN陈剑英,黄文广,陶凯雄,王国斌.CyclinE表达失调导致人类结肠癌CIN. 世界华人消化杂志2006;14(22)2164-2168,CyclinE表达
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陈剑英, 黄文广, 陶凯雄, 王国斌, 华中科技大学同济医学院附属协和医院普外科 湖北省武汉市 430022
陈剑英, 2003年华中科技大学同济医学院博士研究生毕业, 讲师, 主要从事肿瘤外科的基础研究.
国家高科技研究发展计划, No. 2001AA218051
通讯作者: 陈剑英, 430022, 湖北省武汉市解放大道1277号, 华中科技大学同济医学院附属协和医院普外科.
unionchen@yahoo.com.cn.
电话: 027-85726494 传真: 027-85756343
收稿日期: 2006-02-07 接受日期: 2006-06-08
Deregulation of Cyclin E induces chromosomal instability in human colon cancer
Jian-Ying Chen, Wen-Guang Huang, Kai-Xiong Tao, Guo-Bin Wang
Jian-Ying Chen, Wen-Guang Huang, Kai-Xiong Tao, Guo-Bin Wang, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported by the National High Technology Research and Development Program of China, No. 2001AA218051
Correspondence to: Dr. Jian-Ying Chen, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. unionchen@yahoo.com.cn
Received: 2006-02-07 Accepted: 2006-06-08
AbstractAIM:To study the influence of stably inducible expression of Cyclin E on the chromosomal instability in human colon cancer cell line DLD1.
METHODS: Tetracycline-responsive gene-inducible cell line DLD1tTA-cyclin E was generated. Western blot was used to examine the induction of Cyclin E expression upon removal of doxycycline. 4'-6-Diamidino-2-phenylindole (DAPI) staining was performed to detect the percentage of cells with chromosomal instability 1, 3, 5, 7 and 14 d after the induction of Cyclin E expression.
RESULTS: Western blot showed that the peak of Cyclin E expression appeared 96 h after induction. The percentage of cells with chromosomal instability in tet-off DLD1tTA-Cyclin E cells ranged from 0.97% to 1.22% (t = 3.81, P > 0.01). However, the percentages of cells with chromosomal instability were 2 ......
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