幽门螺杆菌重组VacA-HpaA IgY的制备
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叶翠莲，杨致邦，黄 伟, 毛小琴, 张绍兰，黄 进
幽门螺杆菌;重组蛋白;细胞空泡毒素抗原;黏附素;蛋黄抗体,幽门螺杆菌重组VacA-HpaAIgY的制备,叶翠莲,杨致邦,毛小琴,黄进,黄伟,张绍兰,通讯作者:,电话:,传真:,收稿日期:,接受日
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叶翠莲, 杨致邦, 毛小琴, 黄 进, 重庆医科大学微生物学教研究室 四川省重庆市 400016
黄伟, 西南大学蚕学与生物技术学院生物技术系 四川省重庆市 402460
张绍兰, 四川省成都医学院免疫学教研室 四川省成都市 610083
叶翠莲, 2003年重庆医科大学硕士生, 主要从事幽门螺杆菌感染的防治研究.
通讯作者: 杨致邦，400016，重庆市，重庆医科大学基础医学院微生物学教研室. dryangfm365@sina.com
电话: 023-68485090 传真: 023-68486859
收稿日期: 2006-04-24 接受日期: 2006-05-11
Preparation of egg yolk immunoglubin against recombinant vacuolating cytotoxin A-Helicobacter pylori adhesin A in Helicobacter pylori
Cui-Lian Ye, Zhi-Bang Yang, Wei Huang, Xiao-Qin Mao, Shao-Lan Zhang, Jin Huang
Cui-Lian Ye, Zhi-Bang Yang, Xiao-Qin Mao, Jin Huang, Department of Microbiology, Chongqing University of Medical Science, Chongqing 400016, China
Wei Huang, Department of Biology, College of Biotechnology and Silkworm Science, Southwest University, Chongqing 402460, China
Shao-Lan Zhang, Department of Immunology, Chengdu Medical College, Chengdu 610083, Sichuan Province, China
Correspondence to: Professor Zhi-Bang Yang, Department of Microbiology, Chongqing University of Medical Science, Chongqing 400016, China. dryangfm365@sina.com
Received: 2006-04-24 Accepted: 2006-05-11
Abstract
AIM: To prepare a highly specific and efficient egg yolk immunoglubin (IgY) against recombinant vacuolating cytotoxin A-Helicobacter pylori adhesin A (VacA-HpaA) from the yolk of hen’s eggs.
METHODS: Recombinant bacteria of pQE30-VacA-HpaA-DH5α was cultured in large numbers to get VacA-HpaA fusion protein. The recombinant protein was purified by Ni2+-NTA column chromatography and used to immunize the hens. The VacA-HpaA IgY was extracted from the yolk of hen’s eggs by water-dilution methods. In order to evaluate the relationship between IgY titer and immune time, the titer of IgY was detected by enzyme-linked immunosorbent assay (ELISA). IgY was purified and concentrated by deposition technique with ammonium sulfate ......
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