应用SELDI-TOF-MS技术建立肝癌筛选血清蛋白质指纹图谱模型
http://www.100md.com
刘池波, 潘春琴, 孙灵芬
表面增强激光解析电离飞行时间质谱;蛋白芯片;肝癌,刘池波,潘春琴,孙灵芬,通讯作者,Establishmentofserumprot
 |
| 第1页 |
参见附件(843KB，4页)。
刘池波, 潘春琴, 孙灵芬, 浙江省台州市立医院 浙江省台州市 318000
通讯作者: 刘池波, 318000, 浙江省台州市椒江区中山路381号, 浙江省台州市立医院. liuchibo@56.com
电话: 0576-8858213 传真: 0576-8858284
收稿日期: 2006-06-20 接受日期: 2006-07-11
Establishment of serum protein pattern model for screening hepatocellular carcinoma by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry
Chi-Bo Liu, Chun-Qin Pan, Ling-Fen Sun
Chi-Bo Liu, Chun-Qin Pan, Ling-Fen Sun, Taizhou Municipal Hospital, Taizhou 318000, Zhejiang Province, China
Correspondence to:Chi-Bo Liu, Taizhou Municipal Hospital, 381 Zhongshan Road, Jiaojiang District, Taizhou 318000, Zhejiang Province, China. liuchibo@56.com
Received:2006-06-20 Accepted: 2006-07-11
Abstract
AIM: To establish a serum protein pattern model for screening hepatic cellular carcinoma.
METHODS: The proteomic spectra of patients with hepatic cellular carcinoma, hepatic cirrhosis, and healthy people were obtained by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) on WCX2 chips. The collected data were compared and analyzed by Biomarker Wizard software.
RESULTS: A group of proteomic peaks were detected. The expression of five protein molecules (4477, 8943, 5181, 8617, 13 761 Da) in patients with hepatic cellular carcinoma was significantly higher than those in the controls, and the expression of 4477- and 13 761-Da proteins were higher while the 4097-Da one was lower in HCC patients than cirrhosis ones. The specificity and sensitivity of SELDI-TOF-MS were 100% (60/60) and 90% (18/20), respectively. Four protein molecules (4477, 8943, 13 761, 4097 Da) were screened as a proteomic model ......
您现在查看是摘要介绍页，详见PDF附件(843KB，4页)。