L02细胞在2-乙酰氨基芴处理大鼠肝中的增殖
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林 沪, 毛 青, 王宇明, 蒋业贵, 邓国宏,
嵌合肝;L02肝细胞;动物模型;移植;大鼠,林沪,毛青,王宇明,蒋业贵,邓国宏,蒋黎,李俊刚,刘国栋,林沪,通讯作者,Repopulationofhuma
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林沪, 毛青, 王宇明, 蒋业贵, 邓国宏, 蒋黎, 李俊刚, 刘国栋, 中国人民解放军第三军医大学西南医院感染病研究所 重庆市 400038
林沪, 2003年第三军医大学西南医院感染病研究所硕士, 主治医师, 主要从事肝炎发病机制和病毒性肝炎动物模型的研究.
国家自然科基金资助项目, No. 30271173
通讯作者: 毛青, 400038, 重庆市沙坪坝区高滩岩正街30号, 中国人民解放军第三军医大学西南医院感染病研究所. linhu7018@yahoo.com.cn
电话: 023-65399064 传真: 023-68754475
收稿日期: 2006-05-09 接受日期: 2006-06-05
Repopulation of human L02 hepatocytes in tolerant rats treated with 2-acetaminofluorene
Hu Lin, Qing Mao, Yu-Ming Wang, Ye-Gui Jiang, Guo-Hong Deng, Li Jiang, Jun-Gang Li, Guo-Dong Liu
Hu Lin, Qing Mao, Yu-Ming Wang, Ye-Gui Jiang, Guo-Hong Deng, Li Jiang, Jun-Gang Li, Guo-Dong Liu, Institute of Infectious Disease, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China
Supported byNational Natural Science Foundation of China, No. 30271173
Correspondence to:Hu Lin, Institute of Infectious Disease, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China. linhu7018@yahoo.com.cn
Received:2006-05-09 Accepted:2006-06-05
Abstract
AIM: To investigate whether human L02 hepatocytes can repopulate after implantation to the normal, immunocompetent rats treated with 2-acetaminofluorene.
METHODS: Human L02 hepatocytes were injected into the peritoneal cavities of fetal Sprague Dawley rats to induce immune tolerance. The 2-week rats were injected with different doses of 2-acetaminofluorene or normal saline, and received partial hepatoectomy. After 1, 1′-Dioctadecyl-3, 3, 3′, 3′-tetramethylindocarbocyanine perchlorate (DiI) staining, the hepatocytes were implanted to the 3-week rats via the spleen. Immuno-fluorescent staining, reverse transcription-polymerase chain reaction (RT-PCR), S-P immunohistochemistry, and DiI staining were used to detect human albumin, human albumin mRNA and specific proliferating cell nuclear antigen (PCNA) in the rat liver. The distribution of human L02 hepatocytes was observed under fluorescent microscope ......
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